GAD2/GAD65 Antibody [PerCP]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: PerCP

GAD2/GAD65 Antibody [PerCP] Summary

• Immunogen: E. coli-derived recombinant human GAD2/GAD65
  Ala1-Ala150
  Accession # Q05329
• Specificity: Detects human GAD2/GAD65 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human GAD1/GAD67 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GAD2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for GAD2/GAD65 Antibody [PerCP]

• EC 4.1.1
• EC 4.1.1.15
• GAD2
• GAD65
• GAD-65
• GAD65MGC161605
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
• glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• glutamate decarboxylase 2
• Glutamate decarboxylase 65 kDa isoform
• Glutamate decarboxylase-2 (pancreas)
• MGC161607,65 kDa glutamic acid decarboxylase

Background

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD2