GAD1/GAD67 Antibody

Images

 
Western Blot: GAD1/GAD67 Antibody [NBP1-06989] - Staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GAD1/GAD67 Antibody Summary

Immunogen
Peptide with sequence C-PDSPQRREKLHK corresponding to internal region according to NP_000808.2.
Specificity
This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 65 kDa band observed in human brain (cerebral cortex) lysates (calculated MW of 66.9 kDa band according to NP_000808.2). IHC-F usage has been reported in scientific literature (PMID: 24291518), however, results may vary from lot to lot.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
GAD1/GAD67 Lysate (NBL1-10933)
Publications
Read Publication using
NBP1-06989 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine, Porcine. Mouse reactivity reported in scientific literature (PMID: 24291518)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAD1/GAD67 Antibody

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAD1/GAD67 Antibody (NBP1-06989)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


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ICC/IF
(1)
IHC-Fr
(1)
WB
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GAD1/GAD67 Antibody (NBP1-06989) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GAD1
Entrez
Uniprot