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GAD1/GAD67 Antibody (OTI3G9)

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Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1/GAD67 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Immunofluorescent staining of A549 cells using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human pancreas tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1/GAD67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human colon tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human endometrium tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human lung tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human Ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunoprecipitation: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PEP-ELISA
Clone
OTI3G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.46 mg/ml

GAD1/GAD67 Antibody (OTI3G9) Summary

Immunogen
Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 4 ug/mL
  • Peptide ELISA
  • Western Blot 1:1000-2000
Application Notes
Use in PEP-ELISA reported in scientific literature (PMID: 25358241).
Theoretical MW
66.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-48272 in the following applications:

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:33539794). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.46 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAD1/GAD67 Antibody (OTI3G9)

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAD1/GAD67 Antibody (NBP1-48272)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GAD1/GAD67 Antibody (NBP1-48272)

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Diseases for GAD1/GAD67 Antibody (NBP1-48272)

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Bioinformatics

Gene Symbol GAD1
Entrez