GAD1/GAD67 Antibody (GAD1/2391) [Janelia Fluor® 646]

• Reactivity: Hu
• Applications: WB, ELISA, Endotoxin, IHC
• Clone: GAD1/2391
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

GAD1/GAD67 Antibody (GAD1/2391) [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant human GAD1/GAD67 protein fragment (around aa 72-135) (exact sequence is proprietary) (Uniprot: Q99259)
• Localization: Cytoplasmic
• Marker: GABAergic Neuronal Marker
• Specificity: This monoclonal antibody recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAD1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-08276. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GAD1/GAD67 Antibody (GAD1/2391) [Janelia Fluor® 646]

• 67kD)
• EC 4.1.1
• FLJ45882
• GAD1
• GAD25
• GAD67
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD1