FYB/ADAP/SLAP130 Antibody (JE44-80)

Western Blot: FYB/ADAP/SLAP130 Antibody (JE44-80) [NBP2-76882] - Western blot analysis of FYB on U937 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: FYB/ADAP/SLAP130 Antibody (JE44-80) [NBP2-76882] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Flow Cytometry: FYB/ADAP/SLAP130 Antibody (JE44-80) [NBP2-76882] - Flow cytometric analysis of FYB was done on HepG2 cells. The cells were fixed, permeabilized and stained with FYB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

Immunohistochemistry: FYB/ADAP/SLAP130 Antibody (JE44-80) [NBP2-76882] - Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Immunofluorescence: FYB/ADAP/SLAP130 Antibody (JE44-80) [NBP2-76882] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Product Discontinued

FYB/ADAP/SLAP130 Antibody (JE44-80) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human FYB/ADAP/SLAP130 aa 701-750 / 783. (SwissProt: O15117 Human)
• Localization: Cell junction, Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FYB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FYB/ADAP/SLAP130 Antibody (JE44-80)

• ADAP
• Adhesion and degranulation promoting adaptor protein
• adhesion and degranulation-promoting adaptor protein
• FYB
• FYB-120/130SLAP-130FYN-binding protein (FYB-120/130)
• FYN binding protein
• FYN-binding protein
• FYN-T-binding protein
• p120/p130
• PRO0823
• SLAP130
• SLP-76-associated phosphoprotein

Background

FYB acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression ofinterleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FYB1