Fucosyltransferase 3/FUT3 Antibody (SPM194)

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Immunohistochemistry-Paraffin: Fucosyltransferase 3/FUT3 Antibody (SPM194) [NBP2-44597] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Fucosyltransferase 3/FUT3 Antibody (SPM194).

Product Details

Summary
Reactivity Hu, GpSpecies Glossary
Applications IHC, IHC-P, ICC/IF
Clone
SPM194
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Fucosyltransferase 3/FUT3 Antibody (SPM194) Summary

Immunogen
Mucin isolated from a human ovarian cyst fluid
Localization
Cytoplasmic
Specificity
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FUT3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 19p13.3
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Fucosyltransferase 3/FUT3 Antibody (SPM194)

  • CD174
  • FT3B
  • Fucosyltransferase 3
  • FucT-III
  • FUT3
  • Les
  • Lewis FT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47928AF350
Alexa Fluor 405 NBP2-47928AF405
Alexa Fluor 488 NBP2-47928AF488
Alexa Fluor 532 NBP2-47928AF532
Alexa Fluor 594 NBP2-47928AF594
Alexa Fluor 647 NBP2-47928AF647
Alexa Fluor 700 NBP2-47928AF700
Alexa Fluor 750 NBP2-47928AF750
Biotin NBP2-47928B
DyLight 350 NBP2-47928UV
DyLight 405 NBP2-47928V
DyLight 488 NBP2-47928G
DyLight 550 NBP2-47928R
DyLight 594 NBP2-47928DL594
DyLight 650 NBP2-47928C
DyLight 680 NBP2-47928FR
DyLight 755 NBP2-47928IR
FITC NBP2-47928F
HRP NBP2-47928H
Janelia Fluor 549 NBP2-47928JF549
Janelia Fluor 646 NBP2-47928JF646

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Bioinformatics

Gene Symbol FUT3
Uniprot