FTSJ1 Overexpression Lysate (Native)

Western Blot: FTSJ1 Overexpression Lysate (Adult Normal) [NBL1-10853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FTSJ1.

• Reactivity: Hu
• Applications: WB

FTSJ1 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215210 and based on accession number NM_012280. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens FtsJ homolog 1 (E. coli) (FTSJ1), transcript variant 1, mRNA.
• Gene: FTSJ1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for FTSJ1

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FTSJ1 Overexpression Lysate (Native)

• CDLIV
• cell division protein
• EC 2.1.1.-
• FtsJ homolog 1 (E. coli)
• JM23
• mental retardation, X-linked 44
• mental retardation, X-linked 9
• MRX44
• Protein ftsJ homolog 1
• putative ribosomal RNA methyltransferase 1
• rRNA (uridine-2'-O-)-methyltransferase
• SPB1MRX9
• TRM7

Background

The protein encoded by this gene is a member of the S-adenosylmethionine-binding protein family. It is a nucleolar protein and may be involved in the processing and modification of rRNA. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the predominant isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FTSJ1