Reactivity | Hu, Mu, Ch, PmSpecies Glossary |
Applications | WB, IB, IHC |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 1.1 mg/ml |
Immunogen | A synthetic peptide within the C-terminal region (residues 400-505) of the human FTO protein. [Swiss-Prot# Q9C0B1] |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | FTO |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | This FTO antibody is useful for Western blot analysis, where a band is seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in immunoblotting reported in scientific literature (PMID: 22019092). Use in Immunohistochemistry reported in scientific literature (PMID: 28205605). |
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Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Control Peptide |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
Preservative | 0.1% Sodium Azide |
Concentration | 1.1 mg/ml |
Purity | Immunogen affinity purified |
Publications using NB110-60935 | Applications | Species |
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Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods Jan 11 2018 [PMID: 30377371] (Human) Details: Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP). |
Human | |
Walters BJ, Mercaldo V, Gillon CJ et al. The Role of The RNA Demethylase FTO (Fat Mass and Obesity-Associated) and mRNA Methylation in Hippocampal Memory Formation. Neuropsychopharmacology. Mar 15 2017 [PMID: 28205605] | ||
Cloning and characterization of chicken fat mass and obesity associated (Fto) gene: fasting affects Fto expression. Tiwari A, Krzysik-Walker SM, Ramachandran R Domest Anim Endocrinol [PMID: 22019092] (WB, Chicken) | WB | Chicken |
Garcia Baucells P A focus on epitranscriptomics and aging: The effect of m6A (m) modulation on C/EBP beta isoform expression Thesis Jan 1 2022 |
Secondary Antibodies |
Isotype Controls |
Diseases for FTO Antibody (NB110-60935)Discover more about diseases related to FTO Antibody (NB110-60935).
| Pathways for FTO Antibody (NB110-60935)View related products by pathway.
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PTMs for FTO Antibody (NB110-60935)Learn more about PTMs related to FTO Antibody (NB110-60935).
| Research Areas for FTO Antibody (NB110-60935)Find related products by research area.
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Gene Symbol | FTO |