FTO Antibody

Western Blot: FTO Antibody [NB110-60935] - Detection of FTO in human brain lysate using anti-FTO NB110-60935.

• Reactivity: Hu, Mu, Ch, Pm
• Applications: WB, IB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.1 mg/ml

FTO Antibody Summary

• Immunogen: A synthetic peptide within the C-terminal region (residues 400-505) of the human FTO protein. [Swiss-Prot# Q9C0B1]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FTO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunohistochemistry
  • Western Blot 0.5 ug/ml
• Application Notes: This FTO antibody is useful for Western blot analysis, where a band is seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in immunoblotting reported in scientific literature (PMID: 22019092). Use in Immunohistochemistry reported in scientific literature (PMID: 28205605).
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  FTO Peptide (NB110-60935PEP)

Reactivity Notes

Human, mouse and monkey. Chicken reactivity reported in scientific literature (PMID: 22019092).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FTO Antibody

• Alpha-ketoglutarate-dependent dioxygenase FTO
• EC 1.14.11.-
• fat mass and obesity associated
• Fat mass and obesity-associated protein
• FTO
• KIAA1752protein fto
• MGC5149

Background

FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FTO Antibody (NB110-60935)(4)

Publications using NB110-60935

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods Jan 11 2018 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Walters BJ, Mercaldo V, Gillon CJ et al. The Role of The RNA Demethylase FTO (Fat Mass and Obesity-Associated) and mRNA Methylation in Hippocampal Memory Formation. Neuropsychopharmacology. Mar 15 2017 [PMID: 28205605]
• Cloning and characterization of chicken fat mass and obesity associated (Fto) gene: fasting affects Fto expression. Tiwari A, Krzysik-Walker SM, Ramachandran R Domest Anim Endocrinol [PMID: 22019092] (WB, Chicken)
• Garcia Baucells P A focus on epitranscriptomics and aging: The effect of m6A (m) modulation on C/EBP beta isoform expression Thesis Jan 1 2022

FAQs for FTO Antibody (NB110-60935). (Showing 1 - 1 of 1 FAQs).

1. Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
   • With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

Bioinformatics

• Gene Symbol: FTO