FTO Antibody

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Western Blot: FTO Antibody [NB110-60935] - Detection of FTO in human brain lysate using anti-FTO NB110-60935.

Product Details

Summary
Reactivity Hu, Mu, Ch, PmSpecies Glossary
Applications WB, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

FTO Antibody Summary

Immunogen
A synthetic peptide within the C-terminal region (residues 400-505) of the human FTO protein. [Swiss-Prot# Q9C0B1]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FTO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 22019092)
  • Immunohistochemistry reported in scientific literature (PMID 28205605)
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot analysis, a band is seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
FTO Peptide (NB110-60935PEP)
Publications
Read Publications using
NB110-60935 in the following applications:

  • WB
    3 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22019092).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FTO Antibody

  • Alpha-ketoglutarate-dependent dioxygenase FTO
  • EC 1.14.11.-
  • fat mass and obesity associated
  • Fat mass and obesity-associated protein
  • FTO
  • KIAA1752protein fto
  • MGC5149

Background

FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FTO Antibody (NB110-60935)(6)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
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Filter By Species
Human
(3)
Chicken
(1)
All Species

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Product General Protocols

View specific protocols for FTO Antibody (NB110-60935): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FTO Antibody (NB110-60935). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
    • With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for FTO Antibody (NB110-60935)

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Blogs on FTO

There are no specific blogs for FTO, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol FTO