This FTO antibody is useful for Western blot analysis, where a band is seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in immunoblotting reported in scientific literature (PMID: 22019092). Use in Immunohistochemistry reported in scientific literature (PMID: 28205605).
60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse and monkey. Chicken reactivity reported in scientific literature (PMID: 22019092).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for FTO Antibody
Alpha-ketoglutarate-dependent dioxygenase FTO
fat mass and obesity associated
Fat mass and obesity-associated protein
FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for FTO Antibody (NB110-60935). (Showing 1 - 1 of 1 FAQs).
Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.