![Immunohistochemistry-Paraffin: Frizzled-5 Antibody - BSA Free [NBP1-02414] - Staining of human pancreas tissue after heat-induced antigen retrieval. Antibody concentration 7 ug/ml.](http://images.novusbio.com/fullsize2/Frizzled-5-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02414-img0002.jpg "Immunohistochemistry-Paraffin: Frizzled-5 Antibody - BSA Free [NBP1-02414] - Staining of human pancreas tissue after heat-induced antigen retrieval. Antibody concentration 7 ug/ml.")
| Reactivity | Hu, Po, Bv, Ca, Pm, Pm, Rb, Mu, RtSpecies Glossary |
| Applications | ELISA, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Synthetic 14 amino acid peptide from N-terminal extracellular domain of human Frizzled-5. |
| Epitope | N-Terminus extracellular domain |
| Specificity | Human Frizzled-5. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
| Predicted Species | Mouse (93%), Rat (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FZD5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Flow-Cytometry-NBP1-54467-img0012.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.](http://images.novusbio.com/images/beta-Catenin-Antibody-(12F7)-Western-Blot-NBP1-54467-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review.](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Immunohistochemistry-NBP1-54467-img0011.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Analysis of anti-FZD9 / Frizzled 9 antibody with brain, Purkinje neurons at 13 ug/ ml.](http://images.novusbio.com/images2/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Analysis of anti-FZD9 / Frizzled 9 antibody with human brain, glioblastoma.](http://images.novusbio.com/images2/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0003.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5103] - Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/images2/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5103-img0004.jpg)
![Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/images2/HNF-4-alpha-NR2A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89679-img0015.jpg)
![Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining in human duodenum and tonsil tissues using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/HNF-4-alpha-NR2A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89679-img0016.jpg)
![Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/images2/HNF-4-alpha-NR2A1-Antibody-Western-Blot-NBP1-89679-img0017.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0001.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0002.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Frizzled-5 Antibody (NBP1-02414)Discover more about diseases related to Frizzled-5 Antibody (NBP1-02414).
| Pathways for Frizzled-5 Antibody (NBP1-02414)View related products by pathway.
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![Western Blot: Frizzled-2 Antibody [NBP1-20920] - (0.03ug/ml) staining of Human Heart (A) and (0.1ug/ml) Placenta (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/images2/Frizzled-2-Antibody-Western-Blot-NBP1-20920-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Frizzled-2 Antibody [NBP1-20920] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/images2/Frizzled-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20920-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/Frizzled-2-Antibody-Immunohistochemistry-Paraffin-NBP1-20920-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Immunocytochemistry-Immunofluorescence-NBP1-51575-img0006.jpg)
![Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of human LAdrenal tissues using WNT1 mouse mAb.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Immunohistochemistry-Paraffin-NBP1-51575-img0007.jpg)
![Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Western-Blot-NBP1-51575-img0008.jpg)
![Western Blot: Frizzled-10 Antibody [NBP2-23659] - Analysis of Frizzled-10 in human brain lysate. Lane 1 shows pre-immune sera and Lanes 2 show NBP2-23659 tested on human at 1:1000 dilution.](http://images.novusbio.com/images2/Frizzled-10-Antibody-Western-Blot-NBP2-23659-img0001.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")