Frizzled-10 Antibody (1023101) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: IHC
• Clone: 1023101
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Frizzled-10 Antibody (1023101) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line, NS0-derived human Frizzled-10
  Ile21-Gly161
  Accession # Q9ULW2
• Specificity: Detects human Frizzled-10 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-10 Antibody (1023101) [Alexa Fluor® 532]

• CD350 antigen
• CD350
• frizzled (Drosophila) homolog 10
• frizzled homolog 10 (Drosophila)
• Frizzled10
• Frizzled-10
• Fz10
• FZ-10
• FZD10
• FzE7
• hFz10

Background

Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the Frizzled family of Wnt receptors (1, 2). The 205 amino acid N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3 - 5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). PDZ motifs mediate intracellular binding to scaffolding proteins. Within the cysteine-rich domain, human Frizzled-10 shares 71% amino acid (aa) sequence identity with Frizzled-9 and 31% - 46% with Frizzled-1, -2, -3, -4, -5, - 6, -7, and -8. It shares 96%, 94%, 90%, and 82% aa sequence identity with chick, mouse, Xenopus, and zebrafish Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6 - 11). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (12, 13). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 to transduce Wnt-7a and Wnt-7b signals, resulting in the stabilization of cytoplasmic beta-catenin (11, 13). Frizzled-10 is also upregulated in some cancers and transformed cell lines (4, 14). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.