Frequenin Antibody - BSA Free

Western Blot: Frequenin Antibody [NBP3-03316] - Analysis of extracts of various cell lines, using Frequenin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunocytochemistry/ Immunofluorescence: Frequenin Antibody - BSA Free [NBP3-03316] - Immunofluorescence analysis of A549 cells using Frequenin antibody (A13586). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Frequenin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Frequenin Antibody - BSA Free (NBP3-03316) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human Frequenin (NP_055101.2). MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Frequenin Antibody - BSA Free

• DKFZp761L1223
• FREQFLUP
• frequenin (Drosophila) homolog
• frequenin homolog (Drosophila)
• Frequenin homolog
• Frequenin-like protein
• Frequenin-like ubiquitous protein
• NCS-1
• neuronal calcium sensor 1

Background

Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22 kDa when assayed by SDS-PAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. In Drosophila frequenin is related to recoverin and visinin and functions like Ca2+-sensitive guanylyl cyclase activator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCS1