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Frataxin Antibody (FXN/2124)

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Western Blot: Frataxin Antibody (FXN/2124) [NBP2-79802] - Western Blot Analysis of Human Panc-2 and HepG2 cell lysate using Frataxin Antibody (FXN/2124).
Western Blot: Frataxin Antibody (FXN/2124) [NBP2-79802] - Western Blot Analysis of Human Panc-2 and HepG2 cell lysate using Frataxin Antibody (FXN/2124).
Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802] - Formalin-fixed, paraffin-embedded human Pancreas stained with Frataxin Antibody (FXN/2124).
Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) [NBP2-79802] - Formalin-fixed, paraffin-embedded human Pancreas stained with Frataxin Antibody (FXN/2124).
Protein Array: Frataxin Antibody (FXN/2124) [NBP2-79802] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Frataxin Antibody (FXN/2124). Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PA
Clone
FXN/2124
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-79936AF350
Alexa Fluor 405
NBP2-79936AF405
Alexa Fluor 488
NBP2-79936AF488
Alexa Fluor 532
NBP2-79936AF532
Alexa Fluor 594
NBP2-79936AF594
Alexa Fluor 647
NBP2-79936AF647
Alexa Fluor 700
NBP2-79936AF700
Alexa Fluor 750
NBP2-79936AF750
Biotin
NBP2-79936B
DyLight 350
NBP2-79936UV
DyLight 405
NBP2-79936V
DyLight 488
NBP2-79936G
DyLight 550
NBP2-79936R
DyLight 594
NBP2-79936DL594
DyLight 650
NBP2-79936C
DyLight 680
NBP2-79936FR
DyLight 755
NBP2-79936IR
FITC
NBP2-79936F
HRP
NBP2-79936H
Janelia Fluor 549
NBP2-79936JF549
Janelia Fluor 646
NBP2-79936JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-79936
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Frataxin Antibody (FXN/2124) Summary

Immunogen
Recombinant fragment of human Frataxin protein (around aa 57-210) (exact sequence is proprietary)
Localization
Cytoplasmic (Mitochondria)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FXN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Frataxin Antibody (FXN/2124)

  • CyaY
  • EC 1.16.3.1
  • FA
  • FARRmitochondrial
  • frataxin
  • Friedreich ataxia protein
  • Friedreich ataxia
  • Fxn
  • X25MGC57199

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD36 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-79936AF350
Alexa Fluor 405 NBP2-79936AF405
Alexa Fluor 488 NBP2-79936AF488
Alexa Fluor 532 NBP2-79936AF532
Alexa Fluor 594 NBP2-79936AF594
Alexa Fluor 647 NBP2-79936AF647
Alexa Fluor 700 NBP2-79936AF700
Alexa Fluor 750 NBP2-79936AF750
Biotin NBP2-79936B
DyLight 350 NBP2-79936UV
DyLight 405 NBP2-79936V
DyLight 488 NBP2-79936G
DyLight 550 NBP2-79936R
DyLight 594 NBP2-79936DL594
DyLight 650 NBP2-79936C
DyLight 680 NBP2-79936FR
DyLight 755 NBP2-79936IR
FITC NBP2-79936F
HRP NBP2-79936H
Janelia Fluor 549 NBP2-79936JF549
Janelia Fluor 646 NBP2-79936JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol FXN
Uniprot