Frataxin Antibody (3G9) - Azide and BSA Free

Western Blot: Frataxin Antibody (3G9) [H00002395-M03] - Analysis of FXN expression in transfected 293T cell line by FXN monoclonal antibody (M03), clone 3G9. Lane 1: FXN transfected lysatE (23.1 KDa). Lane 2: Non-transfected lysate.

Western Blot: Frataxin Antibody (3G9) [H00002395-M03] - Western blot analysis of FXN over-expressed 293 cell line, cotransfected with FXN Validated Chimera RNAi or non-transfected control. Blot probed with H00002395-M03. GAPDH (36.1 kDa) used as loading control.

• Reactivity: Hu
• Applications: WB, ELISA, Mycoplasma
• Clone: 3G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Frataxin Antibody (3G9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FXN (AAH48097.1, 91 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLAAELTKALKTKLDL
• Specificity: FXN (3G9)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FXN
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi validaton and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Frataxin Antibody (3G9) - Azide and BSA Free

• CyaY
• EC 1.16.3.1
• FA
• FARRmitochondrial
• frataxin
• Friedreich ataxia protein
• Friedreich ataxia
• Fxn
• X25MGC57199

Background

This nuclear gene encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FXN
• Entrez:
  • 2395(Human)
• OMIM:
  • 229300(Human)
• Uniprot:
  • NP_000135(Human)