FoxP1 Antibody (837016) [Unconjugated]

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 25 µg of whole cell lysate (WCL) and 25 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for FoxP1 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FoxP1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

FoxP1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with methanol.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for FoxP1 at approximately 99 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Spinal organoids consists of various spinal cord cell types.Representative images illustrating the presence of a HOXB4+ and b HOXC8+ cells in spinal organoids. Cellular nuclei were counterstained with DAPI. Scale bars, 100 μm. c Co-staining of FOXP1 (green) and ISL1 (red) demonstrates presence of limb-innervating neurons in spinal organoids. Scale bars, 100 μm. d Representative images of spinal organoids at day 42 stained with ISL1 (red) and ChAT (green). Cellular nuclei were counterstained with DAPI. Scale bars, 100 μm. Spinal organoids are stained with e CHX10+ cells (RED) and f CALB+ cells (green). Scale bars, 100 μm. g Co-staining of S100 beta and TUJ1 shows presence of astrocytes in spinal organoids. Scale bars, 100 μm. h Quantitative-PCR analysis demonstrates a lack of dorsal cell types in the spinal organoids generated Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30368521), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
• Clone: 837016
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FoxP1 Antibody (837016) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FoxP1
  Lys548-Glu677
  Accession # Q9H334
• Specificity: Detects human FoxP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP1 Antibody (837016) [Unconjugated]

• FLJ23741
• fork head-related protein like B
• forkhead box P1
• forkhead box protein P1
• FoxP1
• glutamine-rich factor 1
• hFKH1B
• HSPC215
• MGC12942
• MGC88572
• MGC99551
• QRF1
• QRF112CC4

Background

Forkhead Box P1 (FOXP1) is a member of the FOX family of transcription factors. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 contains both a DNA binding domain as well as protein-protein interaction domains. FoxP1 can homo or heterodimerize with FoxP2 and FoxP4, with dimerization necessary for DNA binding. FoxP1 shows both oncogenic and tumor suppressive characteristics. Overexpression in lymphomas leads to poor prognosis, but loss of FoxP1 in breast cancer also implicates a poor prognosis. Human isoforms of 489 to 677 amino acids contain alternate sequences within the first 60 amino acids and/or deletion of amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxP1 Antibody (MAB45341)(3)

Publications using MAB45341

• EP Mulvaney, ÁG O'Sullivan, SB Eivers, HM Reid, BT Kinsella Differential expression of the TP? and TP? isoforms of the human T Prostanoid receptor during chronic inflammation of the prostate: Role for FOXP1 in the transcriptional regulation of TP? during monocyte-macrophage differentiation Exp. Mol. Pathol., 2019-07-02;0(0):104277. 7/2/2019 [PMID: 31271729]
• JH Hor, ES Soh, LY Tan, VJW Lim, MM Santosa, Winanto, BX Ho, Y Fan, BS Soh, SY Ng Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy Cell Death Dis, 2018-10-27;9(11):1100. 2018-10-27 [PMID: 30368521] (IHC, Human)
• O'Sullivan A, Eivers S, Mulvaney E, Kinsella B Regulated expression of the TPbeta isoform of the human T prostanoid receptor by the tumour suppressors FOXP1 and NKX3.1: Implications for the role of thromboxane in prostate cancer. Biochim Biophys Acta Mol Basis Dis, 2017-09-08;1863(12):3153-3169. 2017-09-08 [PMID: 28890397] (Western Blot, Human)

Bioinformatics

• Gene Symbol: FOXP1
• Uniprot:
  • Q9H334(Human)