FoxP1 Antibody (542721)

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB4534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific bands for FoxP1 were detected at approximately 60, 85, and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FoxP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB4534) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 542721
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FoxP1 Antibody (542721) Summary

• Immunogen: E. coli-derived recombinant human FoxP1
  Leu231-Gly420
  Accession # Q9H334
• Specificity: Detects human FoxP1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP1 Antibody (542721)

• FLJ23741
• fork head-related protein like B
• forkhead box P1
• forkhead box protein P1
• FoxP1
• glutamine-rich factor 1
• hFKH1B
• HSPC215
• MGC12942
• MGC88572
• MGC99551
• QRF1
• QRF112CC4

Background

Forkhead Box P1 (FoxP1) is a member of the FOX family of transcription factors. Alternative splicing of human FoxP1 results in multiple transcript variants encoding different isoforms. FoxP1 contains both a DNA binding domain as well as protein‑protein interaction domains. FoxP1 can homo or heterodimerize with FoxP2 and FoxP4, with dimerization necessary for DNA binding and transcriptional activity. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 shows both oncogenic and tumor suppressive characteristics. Human isoforms of 489 to 677 amino acids contain alternate sequences within the first 60 amino acids and/or deletion of amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXP1
• Uniprot:
  • Q9H334(Human)