FOXO4 Antibody

Western Blot: FOXO4 Antibody [NB100-59745] - Staining of K562 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FOXO4 Antibody Summary

• Description: Novus Biologicals Goat FOXO4 Antibody (NB100-59745) is a polyclonal antibody validated for use in WB and ELISA. Anti-FOXO4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EAASQDRMPQDLD corresponding to internal region according to NP_005929.2.
• Epitope: EAASQDRMPQDLD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXO4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/mL
• Control:

  K-562 Whole Cell Lysate
  FOXO4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FOXO4 Antibody

• AFX
• AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7
• Fork head domain transcription factor AFX1
• forkhead box O4
• forkhead box protein O4
• MLLT7MGC120490
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Background

FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXO4 Antibody (NB100-59745)(1)

Publication using NB100-59745

• Paik JH, Kollipara R, Chu G et al. FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis. Cell 2007-01-26 [PMID: 17254969]

Bioinformatics

• Gene Symbol: FOXO4
• Entrez:
  • 4303(Human)
• Uniprot:
  • NP_005929.2(Human)