FOXO4 Antibody

Images

 
Western Blot: FOXO4 Antibody [NB100-59745] - (1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sandwich ELISA: FOXO4 Antibody [NB100-59745] - (0.5ug/ml) as the reporter with NB100-59745 as the capture rabbit antibody (2.5ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, EIA, PEP-ELISA, S-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FOXO4 Antibody Summary

Immunogen
Peptide with sequence C-EAASQDRMPQDLD corresponding to internal region according to NP_005929.2.
Epitope
EAASQDRMPQDLD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXO4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Enzyme Immunoassay 0.5 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Sandwich ELISA 0.5 - 1 ug/ml
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant FOXO4 captured by a rabbit antibody. WB: Approx. 60-65 kDa band observed in K562 lysates (calculated MW of 53.7 kDa band according to NP_005929.2).
Positive Control
K-562 Lysate (NB820-59464)
FOXO4 Lysate (NBL1-10815)
Publications
Read Publication using NB100-59745.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXO4 Antibody

  • AFX
  • AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7
  • Fork head domain transcription factor AFX1
  • forkhead box O4
  • forkhead box protein O4
  • MLLT7MGC120490
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Background

FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOXO4 Antibody (NB100-59745)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FOXO4 Antibody (NB100-59745) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FOXO4 Antibody (NB100-59745)

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Research Areas for FOXO4 Antibody (NB100-59745)

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Blogs on FOXO4.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

The Wise Old Fox: Forkhead Transcription Factors and Age-Related DAF-16 Studies
Orthologs are one of the classes of homolog genes. They occur in different species, but are linked by a common ancestral pathway. During evolution, they retain the same original function, irrespective of the species. Among the orthologs covered on our...  Read full blog post.

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Bioinformatics

Gene Symbol FOXO4
Entrez
Uniprot