FoxF2 Antibody

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxF2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Product Discontinued

FoxF2 Antibody Summary

• Immunogen: E. coli-derived recombinant human FoxF2
  Ala324-Met444
  Accession # Q12947
• Specificity: Detects human FoxF2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) FoxF1, rhFoxP3, and rhFoxC2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FOXF2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxF2 Antibody

• FKHL6
• FKHL6forkhead box protein F2
• forkhead box F2
• forkhead-like 6
• Forkhead-related activator 2
• Forkhead-related protein FKHL6
• Forkhead-related transcription factor 2
• FoxF2
• FREAC2
• FREAC2FREAC-2

Background

FoxF2 (Forkhead box protein F2; also FREAC-2 and FKHL6) is a 42-46 kDa member of the winged helix transcription factor gene family. It is a transcriptional activator that is expressed in embryonic mesenchyme, inducing the synthesis if BMP4 and ECM. In the adult, it is expressed in placenta and lung, specifically in type II pneumocytes plus bronchiolar epithelium, inducing the synthesis of C10 and surfactant proteins B and C. Human FoxF2 is 444 amino acids (aa) in length. It contains one poly-Ala repeat (aa 33-41), a poly-Ser region (aa 46-67), and a poly-Gly segment (aa 77-84), followed by a forkhead DNA binding domain (aa 99-190) and three homopolymeric "runs" involving His (aa 262-272), Gly (aa 301-306) and Ser (aa 312-315). Over aa 324-444, human FoxF2 shares 99% aa identity with mouse FoxF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxF2 Antibody (AF6988)(7)

Publications using AF6988

• D Jia, Z Zhou, OJ Kwon, L Zhang, X Wei, Y Zhang, M Yi, MP Roudier, MC Regier, R Dumpit, PS Nelson, M Headley, L True, DW Lin, C Morrissey, CJ Creighton, L Xin Stromal FOXF2 suppresses prostate cancer progression and metastasis by enhancing antitumor immunity Nature Communications, 2022-11-11;13(1):6828. 2022-11-11 [PMID: 36369237] (IHC, Human)
• Maria-Daniela Cirnaru, Sicheng Song, Kizito-Tshitoko Tshilenge, Chuhyon Corwin, Justyna Mleczko, Carlos Galicia Aguirre et al. Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation eLife 10/5/2021 [PMID: 34609283]
• Maria-Daniela Cirnaru, Sicheng Song, Kizito-Tshitoko Tshilenge, Chuhyon Corwin, Justyna Mleczko, Carlos Galicia Aguirre et al. Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation eLife 2021-10-05 [PMID: 34609283] (Immunohistochemistry, Human)
• Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway Biology Open 7/4/2020 [PMID: 32616504]
• Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway Biology Open 2020-07-04 [PMID: 32616504] (Immunohistochemistry, Mouse)
• J Xu, H Liu, Y Lan, M Adam, DE Clouthier, S Potter, R Jiang Hedgehog signaling patterns the oral-aboral axis of the mandibular arch Elife, 2019-01-14;8(0):. 2019-01-14 [PMID: 30638444] (IHC-P, Mouse)
• Herring BP, Hoggatt AM, Gupta A, Wo JM. Gastroparesis is associated with decreased FOXF1 and FOXF2 in humans, and loss of FOXF1 and FOXF2 results in gastroparesis in mice Neurogastroenterol. Motil. 12/19/2018 [PMID: 30565344]

Bioinformatics

• Gene Symbol: FOXF2
• Uniprot:
  • Q12947(Human)