FOXB1 Antibody (PCRP-FOXB1-1B7)

Immunocytochemistry/Immunofluorescence: FOXB1 Antibody (PCRP-FOXB1-1B7) [NBP3-13926] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using FOXB1 Antibody (PCRP-FOXB1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometry: FOXB1 Antibody (PCRP-FOXB1-1B7) [NBP3-13926] - Flow cytometric analysis of PFA-fixed HeLa cells. FOXB1 antibody (PCRP-FOXB1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: FOXB1 Antibody (PCRP-FOXB1-1B7) [NBP3-13926] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FOXB1 antibody (PCRP-FOXB1-1B7).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-FOXB1-1B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

FOXB1 Antibody (PCRP-FOXB1-1B7) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14021)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human FOXB1protein (Uniprot: Q99853)
• Localization: Nucleus
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for FOXB1 Antibody (PCRP-FOXB1-1B7)

• FKH5HFKH-5
• forkhead box B1
• forkhead box protein B1
• Transcription factor FKH-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXB1
• Uniprot:
  • Q99853(Human)