FOLR1 Antibody [Unconjugated]

FOLR1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

FOLR1 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

FOLR1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse FOLR1
  Arg23-Ser232
  Accession # P35846
• Specificity: Detects mouse FOLR1 in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human FOLR1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FOLR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR1 Antibody [Unconjugated]

• Adult folate-binding protein
• FBP
• folate binding protein
• folate receptor 1 (adult)
• Folate receptor 1
• folate receptor alpha
• Folate receptor, adult
• Folbp1
• FOLR
• FOLR1
• FR-alpha
• KB cells FBP
• MOv18
• Ovarian tumor-associated antigen MOv18

Background

Folate receptor 1 (FOLR1; also folate receptor alpha and folate binding protein 1/FOLBP1) is a 40-50 kDa member of the folate receptor family. FOLR1 is expressed in reticulocytes, retinal Muller cells and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The mouse FOLR1 preproprecursor is 255 amino acid (aa) in length. It contains a GPI-linked glycoprotein that is 208 aa in size (aa 25-232), and which possesses three potential N-linked glycosylation sites. There is one potential isoform variant that shows a 105 aa substitution for aa 110-255. Over aa 23-232, mouse FOLR1 shares 83% and 93% aa sequence identity with human and rat FOLR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOLR1 Antibody (AF6936)(2)

Publications using AF6936

• Buissant des Amorie, JR;Betjes, MA;Bernink, JH;Hageman, JH;Geurts, VE;Begthel, H;Laskaris, D;Heinz, MC;Jordens, I;Vinck, T;Houtekamer, RM;Verlaan-Klink, I;Brunner, SR;van Rheenen, J;Gloerich, M;Clevers, H;Tans, SJ;van Zon, JS;Snippert, HJG; Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients Nature communications 2025-07-22 [PMID: 40695798] (Immunohistochemistry, Mouse)
• C Karampelia, H Rezanejad, M Rosko, L Duan, J Lu, L Pazzagli, P Bertolino, CE Cesta, X Liu, GS Korbutt, O Andersson Reinforcing one-carbon metabolism via folic acid/Folr1 promotes &beta-cell differentiation Nature Communications, 2021-06-07;12(1):3362. 2021-06-07 [PMID: 34099692] (IHC, Mouse)

Bioinformatics

• Gene Symbol: FOLR1
• Uniprot:
  • P35846()