Follistatin-like 1/FSTL1 Antibody

Western Blot: Follistatin-like 1/FSTL1 Antibody [NB100-1095] - Staining of Human Ovary lysate (A) + peptide (B) (35ug protein in RIPA buffer) using 1 ug/ml of this antibody. Detected by chemiluminescence.

Western Blot: Follistatin-like 1/FSTL1 Antibody [NB100-1095] - (0.3ug/ml) staining of Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Follistatin-like 1/FSTL1 Antibody [NB100-1095] - Analysis of FSTL1 in Human placenta lysate using this antibody at 1 ug/ ml. Primary incubated for 1 hour. Detected using chemiluminescence.

• Reactivity: Hu, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Follistatin-like 1/FSTL1 Antibody Summary

• Immunogen: Peptide with sequence C-TAEKTKRVSTKEI corresponding to C-Terminus according to NP_009016.1.
• Localization: Secreted
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FSTL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Experiments gave bands at ~ 55 kDa band, 40 kDa band in human placenta lysate after 0.3 ug/ml antibody staining. Experiments gave bands at ~ 50 kDa band in human placenta lysate after 1 ug/ml antibody staining and in human ovary lysate after 3 ug/ml antibody staining. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 9786430). This protein has a calculated MW of 35.0 kDa band according to NP_009016.1.
• Control:

  Human Ovary Whole Tissue Lysate (Adult Whole Normal)
  Follistatin-like 1/FSTL1 Overexpression Lysate
  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Follistatin-like 1/FSTL1 Peptide (NB100-1095PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Follistatin-like 1/FSTL1 Antibody

• Flik
• FLJ50214
• Follistatin-like 1
• Follistatin-like protein 1
• follistatin-related protein 1
• Follistatin-related Protein
• FRP
• FRPFLJ52277
• FSL1
• FSTL1
• TSC-36

Background

FSTL1 may modulate the action of some growth factors on cell proliferation and differentiation and also binds heparin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-like 1/FSTL1 Antibody (NB100-1095)(1)

Publication using NB100-1095

• Tanaka M, Ozaki S, Osakada F et al. Cloning of follistatin-related protein as a novel autoantigen in systemic rheumatic diseases. Int Immunol 1998-09-01 [PMID: 9786430]

Bioinformatics

• Gene Symbol: FSTL1
• Entrez:
  • 11167(Human)
• Uniprot:
  • NP_009016.(Human)