FMRP Antibody

Western Blot: FMRP Antibody [NBP1-51944] - NBP1-51944 (0.3ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FMRP Antibody Summary

• Description: Novus Biologicals Goat FMRP Antibody (NBP1-51944) is a polyclonal antibody validated for use in WB and ELISA. Anti-FMRP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NPNKPATKDTFHKIK corresponding to internal region according to NP_002015.1.
• Epitope: C-NPNKPATKDTFHKIK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FMR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in lysates of HeLa, Jurkat and Kelly (calculated MW of 71.2 kDa band according to NP_002015.1). IHC: An anonymous customer has reported found nuclear staining in selected trophoblasts of human term placenta.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FMRP Antibody

• FMRPFRAXAPOF
• fragile X mental retardation 1 protein
• fragile X mental retardation 1
• MGC87458
• POF1
• premature ovarian failure 1
• Protein FMR-1

Background

Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyze the role of the FXR family and to identify genes in the FMRP pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FMRP Antibody (NBP1-51944)(1)

Publication using NBP1-51944

• Dolen G, Osterweil E, Rao BS et al. Correction of fragile X syndrome in mice. Neuron 2007-12-20 [PMID: 18093519]

Bioinformatics

• Gene Symbol: FMR1
• Entrez:
  • 2332(Human)
  • 24948(Rat)
• Uniprot:
  • NP_002015.1(Human)