FMRP Antibody

Western Blot: FMRP Antibody [NBP1-20918] - (0.1ug/ml) staining of KELLY cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FMRP Antibody Summary

• Description: Novus Biologicals Goat FMRP Antibody (NBP1-20918) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-FMRP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RTGKDRNQKKEKPD corresponding to internal region (near C-Terminus) according to NP_002015.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FMR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in KELLY lysate is reproted in scientific literature (PMID: 8789445).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FMRP Antibody

• FMRPFRAXAPOF
• fragile X mental retardation 1 protein
• fragile X mental retardation 1
• MGC87458
• POF1
• premature ovarian failure 1
• Protein FMR-1

Background

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FMRP Antibody (NBP1-20918)(1)

Publication using NBP1-20918

• Hanson JE, Blank M, Valenzuela RA et al. The functional nature of synaptic circuitry is altered in area CA3 of the hippocampus in a mouse model of Down's syndrome. J Physiol 2007-02-15 [PMID: 17158177]

Bioinformatics

• Gene Symbol: FMR1
• Entrez:
  • 2332(Human)
  • 14265(Mouse)
  • 24948(Rat)
• Uniprot:
  • Q06787(Human)