Human Flt-3 Ligand/FLT3L Recombinant Protein

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Concentration: LYOPH

Human Flt-3 Ligand/FLT3L Recombinant Protein Summary

• Description: A single glycosylated polypeptide chain protein corresponding to amino acids Gly27 - Arg188 of FLT3LG.
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in 50% acetonitrile and 0.1% TFA.
• Details of Functionality: The ED50, as determined by a cell proliferation assay using Flt-3 transfected murine BaF3 cells, is less than 4 ng/ml.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: FLT3LG
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/mg of the rMuFlt-3 Ligand as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: Between 18-32 due to varying glycosylation kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Reconstitute with PBS to a final concentration of 0.05 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human Flt-3 Ligand/FLT3L Recombinant Protein

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand promotes the differentiation of multiple hematopoietic cell lineages. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells, B cell differentiation, NK cell development, and myeloid differentiation of hematopoietic stem cells. The mouse and human proteins show cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FLT3LG