Flt-3 Ligand/FLT3L Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Flt-3 Ligand/FLT3L Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Flt-3 Ligand/FLT3L
  Gly27-Arg188
  Accession # P49772
• Specificity: Detects mouse Flt-3 Ligand/FLT3L in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Flt-3 Ligand/FLT3L is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Flt3l
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Flt-3 Ligand Antibody (Catalog # AF427)
  ELISA Detection: Mouse Flt-3 Ligand Biotinylated Antibody (Catalog # BAF427)
  Standard: Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Flt-3 Ligand/FLT3L Antibody [Biotin]

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1‑3). Mature mouse Flt-3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4‑6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. Mouse and human Flt-3 Ligand show cross-species activity (4, 5, 7). Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine domain (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3‑6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 11). Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation (12).

1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
4. Hannum, C. et al. (1994) Nature 368:643.
5. Lyman, S.D. et al. (1993) Cell 75:1157.
6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
7. Lyman, S.D. et al. (1994) Blood 83:2795.
8. McClanahan, T. et al. (1996) Blood 88:3371.
9. Lyman, S.D. et al. (1995) Oncogene 10:149.
10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3 Ligand/FLT3L Antibody (BAF427)(4)

Publications using BAF427

• Kohei Fujita, Svetoslav Chakarov, Tetsuro Kobayashi, Keiko Sakamoto, Benjamin Voisin, Kaibo Duan et al. Cell-autonomous FLT3L shedding via ADAM10 mediates conventional dendritic cell development in mouse spleen Proceedings of the National Academy of Sciences 2019-07-16 [PMID: 31262819]
• LM Hillyer, B Woodward Acutely malnourished weanling mice administered Flt3 ligand can support a cell-mediated inflammatory response Cytokine, 2018-07-07;113(0):39-49. 2018-07-07 [PMID: 30539781] (ELISA Development (Detection), Human)
• JR Lee, I Appelmann, C Miething, TO Shultz, D Ruderman, D Kim, P Mallick, SW Lowe, SX Wang Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors Theranostics, 2018-02-03;8(5):1389-1398. 2/3/2018 [PMID: 29507628]
• Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Flt3l
• Uniprot:
  • P49772()