Human Flt-3 Ligand ELISA Kit (Colorimetric)

Images

 
ELISA: Human Flt-3 Ligand ELISA Kit (Colorimetric) [KA1716] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum, Urine
Sensitivity
1.5 pg/mL

Order Details

Human Flt-3 Ligand ELISA Kit (Colorimetric) Summary

Sensitivity
1.5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Spike Recovery
Serum: 95.48%
Plasma: 94.39%
Cell culture media: 97.62%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FLT3LG

Applications/Dilutions

Application Notes
FLT3LG (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FLT3LG.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. HRP-Streptavidin Concentrate (Item G): 600x concentratedHRP-conjugated streptavidin.
  2. Flt-3L Microplate (Item A): coated with anti-human Flt-3L
  3. Detection Antibody Flt-3L (Item F): biotinylated anti-human Flt-3L (eachvial is enough to assay half microplate)
  4. Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium/urine) diluent.
  5. Assay Diluent A (Item D): animal serum with 0.09% sodium azide aspreservative. For Standard/Sample (serum/plasma) diluent.
  6. Stop Solution (Item I): 0.2 M sulfuric acid.
  7. Standards (Item C): recombinant human Flt-3L
  8. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Flt-3 Ligand ELISA Kit (Colorimetric)

  • FL
  • Flt3 ligand
  • Flt-3 Ligand
  • Flt3L
  • FLT3LG
  • fms-related tyrosine kinase 3 ligand
  • SL cytokine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FLT3LG
Entrez