Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) [NB110-94155] - Flt-3 antibody staining in mouse lung cancer tissue. IHC-P image submitted by a verified customer review.

Immunohistochemistry: Flt3/CD135 Antibody (12B6) [NB110-94155] - Paraffin kidney and lung sections from LPS treated mice were subjected to IHC using anti Flt3/CD135 Antibody (12B6) RM0021-12B6

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 12B6
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free Summary

• Immunogen: Mouse Flt-3/Flk-2/CD135 (extracellular domain)
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: FLT3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:100-1:1000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

• CD135 antigen
• CD135
• EC 2.7.10
• fetal liver kinase 2
• FL cytokine receptor
• FLK2
• Flk-2
• FLT3 receptor tyrosine kinase
• Flt3
• Flt-3
• Fms-like tyrosine kinase 3
• fms-related tyrosine kinase 3
• growth factor receptor tyrosine kinase type III
• Stem cell tyrosine kinase 1
• STK-1
• STK1EC 2.7.10.1
• Tyrosine-protein kinase receptor FLT3

Background

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Flt-3/Flk-2/CD135 Antibody (NB110-94155) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 11/30/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Lung cancer tissue
• Species: Mouse
• Lot: 12B6

Comments

• Comments: 4% formaldehyde fixed paraffin sections were processed in dilutions of ethanol. 1ry antibody dilution was 1:100, 2ry antibody 1:400.

Bioinformatics

• Gene Symbol: FLT3
• Entrez:
  • 2322(Human)