Flotillin-1 Antibody (JB19-45)

Western Blot: Flotillin-1 Antibody (JB19-45) [NBP2-75492] - Analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Flotillin-1 Antibody (JB19-45) [NBP2-75492] - Analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Flotillin-1 Antibody (JB19-45) [NBP2-75492] - Analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Flotillin-1 Antibody (JB19-45) [NBP2-75492] - Analysis of paraffin-embedded human lung cancer tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Flotillin-1 Antibody (JB19-45) [NBP2-75492] - Analysis of paraffin-embedded human liver tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Flotillin-1 Antibody (JB19-45) [NBP2-75492] - Analysis of paraffin-embedded human spleen tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.

Product Discontinued

Flotillin-1 Antibody (JB19-45) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Flotillin-1 aa 378-427 / 427. (SwissProt: O75955 Human; SwissProt: O08917 Mouse; SwissProt: Q9Z1E1 Rat)
• Localization: Cell membrane. Endosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FLOT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Flotillin-1 Antibody (JB19-45)

• flotillin 1
• flotillin-1
• integral membrane component of caveolae

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLOT1