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FKBP9 Antibody

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Western Blot: FKBP9 Antibody [NBP1-83887] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: FKBP9 Antibody [NBP1-83887] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: FKBP9 Antibody [NBP1-83887] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FKBP9 Antibody [NBP1-83887] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FKBP9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TGMDQALVGMCVNERRFVKIPPKLAYGNEGVSGVIPPNSVLHFDVLLMDIWNSEDQVQIHTYFKPPSCPRTIQVSDF
Specificity
Specificity of human FKBP9 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FKBP9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FKBP9 Protein (NBP1-83887PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FKBP9 Antibody

  • 63 kDa FK506-binding protein
  • DKFZp586B1723
  • FK506 binding protein 9, 63 kDa
  • FKBP60
  • FKBP63
  • FKBP-9
  • MGC126772,63 kDa FKBP
  • MGC138258
  • peptidyl-prolyl cis-trans isomerase FKBP9
  • PPIase
  • rotamase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclophilin A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclophilin A at approximately 25 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
PPIL1 Antibody
NBP2-57062
Western Blot: PPIL1 Antibody [NBP2-57062] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/Immunofluorescence: PPIL1 Antibody [NBP2-57062] - Staining of human cell line SK-MEL-30 shows localization to nucleoli.
Species: Hu
Applications: WB, ICC/IF
Cyclophilin A
Antibody [Unconjugate
AF3589
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclophilin A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclophilin A at approximately 25 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
FKBP12 Antibody
NB300-508
Western Blot: FKBP12 Antibody [NB300-508] - Analysis of purified FKBP12 after immunoprecipatation on a 15% gel.
Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Colon carcinoma tissue.
Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Heart tissue.
Species: Hu, Mu, Rt, Po
Applications: WB, IHC, IHC-P, IP
PDZD2/PDZK3
Antibody (787924)
MAB7670
Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PDZD2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDZD2 was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu
Applications: WB, ICC
Pin1 Antibody
(257417)
MAB2294
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pin1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Pin1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications: WB, IHC, IP
FKBP52/FKBP4
Antibody
NBP1-84675
Western Blot: FKBP52/FKBP4 Antibody [NBP1-84675] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining in human testis and duodenum tissues using anti-FKBP4 antibody. Corresponding FKBP4 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
FKBP51/FKBP5
Antibody
NBP1-84676
Western Blot: FKBP51/FKBP5 Antibody [NBP1-84676] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining in human breast and cerebral cortex tissues using anti-FKBP5 antibody. Corresponding FKBP5 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Cyclophilin 40
Antibody
NB120-3562
Western Blot: Cyclophilin 40 Antibody [NB120-3562] - Analysis of rat spleen extracts.
Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody [NB120-3562] - Analysis of Cyclophilin D in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cyclophilin D polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cyclophilin D staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NB120-3562] - Both normal and cancer biopsies of deparaffinized human Hepatocarcinoma tissue.
Species: Hu, Mu, Rt, Bv, Ch, Pm, Rb
Applications: WB, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
PPIG Antibody
NBP1-19117
Western Blot: PPIG Antibody [NBP1-19117] - Whole cell lysate from HeLa cells. SRcyp was also immunoprecipitated by rabbit anti-SRcyp antibody NBP1-19116.
Immunohistochemistry-Paraffin: PPIG Antibody [NBP1-19117] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-SRcyp used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
Immunohistochemistry: PPIG Antibody [NBP1-19117] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SRcyp used at a dilution of 1:200 (1ug/ml). Detection: DAB
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P, IP
HSP90 alpha
Antibody
NB120-2928
Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP86 polyclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
(7 Publications)
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
Cyclophilin C
Antibody (3C6)
NBP1-97946
Western Blot: Cyclophilin C Antibody (3C6) [NBP1-97946] - Analysis in HeLa, 1:1000 dilution.
Immunocytochemistry/Immunofluorescence: Cyclophilin C Antibody (3C6) [NBP1-97946] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPIC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Cyclophilin C Antibody (3C6) [NBP1-97946] - Analysis of PPIC in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
FKBP13/FKBP2
Antibody
NBP2-38604
Western Blot: FKBP13/FKBP2 Antibody [NBP2-38604] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: FKBP13/FKBP2 Antibody [NBP2-38604] - Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane.
Immunohistochemistry-Paraffin: FKBP13/FKBP2 Antibody [NBP2-38604] - Staining in human epididymis and skeletal muscle tissues using anti-FKBP2 antibody. Corresponding FKBP2 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MIPEP Antibody
(OTI5E7)
NBP2-45861
Western Blot: MIPEP Antibody (5E7) [NBP2-45861] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIPEP.
Immunohistochemistry: MIPEP Antibody (5E7) [NBP2-45861] - Analysis of Carcinoma of Human thyroid tissue.
Flow Cytometry: MIPEP Antibody (5E7) [NBP2-45861] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MIPEP antibody.
Species: Hu
Applications: WB, Flow, IHC, IF
Aquaporin-0
Antibody
NBP2-58661
Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-58661] - Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in a subset of hepatocytes.
Species: Hu
Applications: IHC, IHC-P
Inorganic
Pyrophosphatase/PPA1
NBP1-31348
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract.
Immunocytochemistry/Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Transportin 1
Antibody (D45)
NB600-1397
Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Transportin 1 (D45) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.
Flow (Intracellular): Transportin 1 Antibody (D45) [NB600-1397] - HeLa cells were stained with Transportin 1 NB600-1397 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.
(5 Publications)
Species: Hu, Bv, Ca, Mk, Rb
Applications: WB, Flow, IHC, IHC-P, IP, MiAr, CyTOF-ready, Flow-IC
PPIL2 Antibody
NBP1-85360
Western Blot: PPIL2 Antibody [NBP1-85360] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: PPIL2 Antibody [NBP1-85360] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PPIL2 Antibody [NBP1-85360] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Cyclophilin-F
Antibody
NBP2-15079
Western Blot: Cyclophilin-F Antibody [NBP2-15079] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Cyclophilin-F Antibody [NBP2-15079] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Cyclophilin-F Antibody [NBP2-15079] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
FKBP9 Antibody
NBP1-83887
FKBP9 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol FKBP9