Cyclophilin C Antibody (3C6)

Images

 
Western Blot: Cyclophilin C Antibody (3C6) [NBP1-97946] - Analysis in HeLa, 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: Cyclophilin C Antibody (3C6) [NBP1-97946] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPIC antibody (1:100) with goat ...read more
Flow Cytometry: Cyclophilin C Antibody (3C6) [NBP1-97946] - Analysis of PPIC in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
3C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Cyclophilin C Antibody (3C6) Summary

Immunogen
Recombinant human PPIC (39-197aa) protein purified from E. coli
Specificity
Anti-human PPIC mAb, clone 3C6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPIC protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPIC
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Cyclophilin C Antibody (3C6)

  • Cyclophilin C
  • CYPCMGC3673
  • EC 5.2.1.8
  • parvulin
  • peptidyl-prolyl cis-trans isomerase C
  • peptidylprolyl isomerase C (cyclophilin C)
  • PPIase C
  • Rotamase C

Background

PPIC is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPIC
Uniprot