FKBP51/FKBP5 Antibody (OTI3E9)

Western Blot: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP5/FKBP51 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP5/FKBP51.

Immunocytochemistry/Immunofluorescence: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FKBP5/FKBP51.

Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded prostate tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded colon tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded endometrium tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded pancreas tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI3E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FKBP51/FKBP5 Antibody (OTI3E9) Summary

• Description: Novus Biologicals Mouse FKBP51/FKBP5 Antibody (OTI3E9) (NBP1-47755) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human FKBP5 expression vector
• Specificity: This antibody is specific for Homo sapiens FK506 binding protein 5 (FKBP5), transcript variant 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FKBP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 51.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FKBP51/FKBP5 Antibody (OTI3E9)

• 51 kDa FKBP
• 54 kDa progesterone receptor-associated immunophilin
• AIG6,51 kDa FK506-binding protein
• Androgen-regulated protein 6
• Dit1
• FF1 antigen
• FK506 binding protein 5
• FK506-binding protein 5
• FKBP5
• FKBP-5
• FKBP51
• FKBP-51
• FKBP51PPIase FKBP5
• FKBP54
• FKBP54EC 5.2.1.8
• HSP90-binding immunophilin
• MGC111006
• p54
• P54,51 kDa FK506-binding protein 5
• peptidyl-prolyl cis-trans isomerase FKBP5
• peptidylprolyl cis-trans isomerase
• PPIase
• Ptg-10
• rotamase
• T-cell FK506-binding protein

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition.It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FKBP5
• Entrez:
  • 2289(Human)