FKBP25 Antibody

Western Blot: FKBP25 Antibody [NBP3-18658] - Figure 2. Western blot analysis using the antibody directed against FKBP25 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse FKBP25 , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 25 kDa) is indicated on the right.

ELISA: FKBP25 Antibody [NBP3-18658] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse FKBP25 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,400.

• Reactivity: Mu
• Applications: WB, Simple Western, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FKBP25 Antibody Summary

• Description: Novus Biologicals Rabbit FKBP25 Antibody (NBP3-18658) is a polyclonal antibody validated for use in IHC, WB, ELISA and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against mouse FKBP25 (FK506 Binding Protein 3), using a KLHconjugated synthetic peptide containing an amino acid sequence from the central part of the protein (1).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FKBP3
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Simple Western 1:25
  • Western Blot 1:500
• Application Notes: See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:25

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for FKBP25 Antibody

• EC 5.2.1.8,25 kDa FK506-binding protein
• FK506 binding protein 3, 25kDa
• FK506-binding protein 3 (25kD)
• FK506-binding protein 3
• FKBP25
• FKBP25,25 kDa FKBP
• FKBP3
• FKBP-3
• Immunophilin FKBP25
• peptidyl-prolyl cis-trans isomerase FKBP3
• PPIase FKBP3
• PPIase
• rapamycin binding protein
• Rapamycin-selective 25 kDa immunophilin
• rotamase
• T-cell

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FKBP3
• Uniprot:
  • Q00688()