FKBP15 Antibody

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Western Blot: FKBP15 Antibody [NB100-423] - Detection of Human FKPB-135 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Western Blot: FKBP15 Antibody [NB100-423] - Detection of human FKBP-135 in Western Blot and immunoprecipitation, using NB100-423. Samples: HeLa nuclear extracts. Exposure times of (A) 25 minutes and (B) 10 minutes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

FKBP15 Antibody Summary

Immunogen
A synthetic peptide mapping to a region between residues 960 and 1020 of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FKBP15
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2 - 10 ug/mg of lysate
Application Notes
Western Blot and immunoprecipitation. Immunohistochemistry has not been tested. *The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FKBP15 Antibody

  • FK506 binding protein 15, 133kDa
  • FK506-binding protein 133kDa
  • FK506-binding protein 15
  • FKBP133
  • FKBP-133
  • FKBP-15,133 kDa FKBP
  • PPP1R76,133 kDa FK506-binding protein
  • regulatory subunit 76
  • WAFL

Background

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FKBP15
Entrez
Uniprot