Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.

ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - ELISA results of purified Fibroblast Activation protein alpha/FAP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Fibroblast Activation Protein alpha/FAP Antibody - BSA Free Summary

• Description: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Fibroblast Activation Protein alpha/FAP Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Fibroblast Activation Protein alpha/Fibroblast Activation Protein alpha/FAP protein. (Uniprot: P97321)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:20000
  • Flow Cytometry Validated for Flow Cytometry from a verified customer review.
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with Fibroblast Activation Protein alpha/FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with Fibroblast Activation Protein alpha/FAP from other sources has not been determined. Human reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Activation Protein alpha/FAP Antibody (NB110-85534)(4)

Publications using NB110-85534

• Ye Z, Pan J, Yin Z et al. Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-? and human livin ? exert an antitumor effect against Lewis lung carcinoma in mice Immun Inflamm Dis 2023-09-27 [PMID: 37773704] (Western Blot)
• Li X, Yong T, Wei Z et al. Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts Nature communications 2022-05-19 [PMID: 35589680] (WB, Mouse)
• Xie J, Yuan S, Peng L et al. Antitumor immunity targeting fibroblast activation protein‑ alpha in a mouse Lewis lung carcinoma model Oncol Lett 2020-05-18 [PMID: 32566014] (WB, Mouse)
• Zhou Z, Zhou Q, Wu X, et al. VCAM-1 Secreted from Cancer-Associated Fibroblasts Enhances the Growth and Invasion of Lung Cancer Cells through AKT and MAPK Signaling Cancer Letters 2020-01-01 [PMID: 31904479] (WB)

Bioinformatics

• Gene Symbol: FAP
• Entrez:
  • 2191(Human)
  • 14089(Mouse)
  • 192203(Rat)
• Uniprot:
  • P97321()
  • P97321()
  • P97321()
  • P97321()
  • Q12884()