Fibroblast Activation Protein alpha/FAP: Novus Biologicals

AF3715

Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

(1 Review)

100 μg

AF3715

HAF016). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Fibroblast Activation Protein a/FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of IMR‑90 human lung fibroblast cell line and WI‑38 human lung fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 130 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF016). A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 97 kDa (as indicated) in the parental WI‑38 human lung fibroblast cell line, but is not detectable in knockout WI‑38 human lung fibroblast cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of WI‑38 human lung fibroblast cell line and human FAP knockout WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 97 kDa (as indicated) in the parental WI‑38 human lung fibroblast cell line, but is not detectable in knockout WI‑38 human lung fibroblast cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

AF3715) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of human squamous cell carcinoma in the esophagus were probed for FAP mRNA (ACD RNAScope Probe, catalog # 411971; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using sheep anti-human FAP polyclonal antibody (R&D Systems catalog # <a class=

AF3715) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. " class="big_thumb" />

FGFR2 drives the process of liver fibrosis. (A) The expression of liver fibrosis markers (ACTA2, fibroblast activation protein alpha (FAP), alpha-1 type I collagen (COL1A1)) was measured by qPCR. The samples were divided into four groups according to whether cells were induced with or without TGF-beta and whether cells were overexpressed by FGFR2. (B) The expression of markers in wild-type and FGFR2-OE cell lines under TGF-beta induction was analyzed by Western blot analysis. (C) The expression levels of alpha -SMA and (D) collagen secretion in LX-2 and Huh-7 were evaluated, when FGFR2 was overexpressed or knocked down upon equal TGF-beta induction. (E) Cells were collected after 48 h of co-culture, and the expression of alpha -SMA and (F) type I collagen in the lower layer cells was determined by ELISA. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

CYN blocks the activation and development of liver fibrosis in vitro. (A) Evaluation of the inhibitory effect of CYN on the fibrosis-promoting effect of FGFR2. The fibrotic transformation of wild-type and FGFR2-OE LX-2 cells and Huh-7 cells was induced through TGF-beta activation, followed by an intervention with CYN for the relevant groups. The expression changes of the fibrosis markers ACTA2 and COL1A1 were analyzed by qPCR. (B) Wild-type cell lines were employed in the aforementioned experiments, and the activation of FGFR2 was triggered by supplementation with the exogenous basic fibroblast growth factor (bFGF) factor. (C) Analysis of the extent of antagonism of CYN towards TGF-beta Signaling. Activation induction models were established by adding or not adding TGF-beta to the cell culture environment with or without the CYN intervention, and the expression of liver fibrosis markers was determined by qPCR, (D) Western blot, and (E,F) ELISA analyses. (G) A co-culture model was used to evaluate the blocking effect of CYN on liver fibrosis activation of signaling transmission. The activation intensity of lower-layer wild-type cells was measured and compared using alpha -SMA expression and collagen secretion. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

High expression of fibroblast growth factor receptor 2 (FGFR2) coincides with liver fibrosis. (A) The expression of FGFR2 in fibrotic liver and normal liver tissues was evaluated through data mining of human samples affected by Hepatitis B infection (GSE38941), alcohol abuse (GSE28619), and nonalcoholic steatohepatitis (GSE48452), as well as mouse samples induced with carbon tetrachloride (CCl4) (GSE152329, GSE98577). The differential fold expression was determined by comparing the expression of FGFR2 in the fibrotic liver and normal liver tissues. (B) A comparison of changes in FGFR2 expression in liver fibrosis patients in remission (improved) or not in remission (not improved) with mining data of GSE175448. (C) The correlation between FGFR2 expression and the liver fibrosis score (Ishak score) was analyzed by comparing FGFR2 expression in three pairs of liver fibrotic tissues and normal liver tissues through immunohistochemistry. (D) FGFR2 expression in liver fibrosis tissues and normal liver tissues was verified by Western blot and (E) q-PCR analyses. (E) Liver tissues from mice treated with CCl4 for different durations were used to determine the expression of the liver fibrosis markers Actin Alpha 2 (ACTA2) and FGFR2 by q-PCR and the trends of their expression with increasing days of induction. (F) The expression of ACTA2 and (G) FGFR2 was determined by q-PCR in liver tissues from mice treated with CCl4 for varying durations. The statistical significance was determined based on the p-value. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, “***” when p < 0.001, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression analysis of Fap in OA synovium. a Immunostaining of human FAP in the synovium of control and OA patients. DAPI staining indicates the nucleus. Scale bars: 100 μm. b qPCR analysis of human FAP mRNA levels in the synovium of control and OA patients (n = 8 samples per group). c Western blot analysis of human FAP protein levels in the synovium of control and OA patients (n = 3 samples per group). d Immunostaining of mouse Fap in the knee joint of sham and DMM-treated mice. Sham or DMM surgery was performed in 8-week-old FapLacZ/+ mice, which were sacrificed 8 weeks later, and LacZ immunostaining was used to detect Fap expression at the posterior horn of the medial meniscus (F: femur; T: tibia; M: meniscus; S: synovium). DAPI staining indicates the nucleus. Scale bars: 100 μm. The statistical significance was assessed using two-tailed Student’s unpaired t tests. Data are presented as the mean ± SD (**P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36588124), licensed under a CC-BY license. Not internally tested by R&D Systems.

AREG promotes migration and CAF-like differentiation of MSCs: (A,B) To investigate the differentiation of MSCs into CAFs upon treatment with rhAREG (100 ng/mL), the mRNA and protein expression levels of FAP, IL-6, and alpha SMA were compared using qRT-PCR (A) and Western blotting (B). beta -actin was used as a loading control (B). (C,D) The effects of rhAREG (100 ng/mL) on the survival (C) and growth (D) of MSCs were evaluated using the MTS assay. (E) The effect of rhAREG (100 ng/mL) on the migration of MSCs was evaluated using the transwell migration assay. MSCs were seeded in the upper chamber, and migrated cells were counted in five fields of view after 48 h. Representative images for each condition are presented below the graph (E). The data are presented as the mean ± SEM of three independent experiments (A,C–E). N.S., not significant; ** p < 0.01, *** p < 0.001. Scale bars: 100 μm (E). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39451251), licensed under a CC-BY license. Not internally tested by R&D Systems.

3715-SE) was serially diluted and captured by Mouse Anti-Human Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB3715) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Fibroblast Activation Protein alpha /FAP (Catalog # <a class=

3715-SE) was serially diluted and captured by Mouse Anti-Human Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB3715) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3715U-100UG
Alexa Fluor 405	AF3715V-100UG
Alexa Fluor 488	AF3715G-100UG
Alexa Fluor 532	AF3715X-100UG
Alexa Fluor 594	AF3715T-100UG
Alexa Fluor 647	AF3715R-100UG
Alexa Fluor 700	AF3715N-100UG
Alexa Fluor 750	AF3715S-100UG
Alexa Fluor Plus 405	AF3715AFP405-100UG
Alexa Fluor Plus 488	AF3715AFP488-100UG
Alexa Fluor Plus 555	AF3715AFP555-100UG
Alexa Fluor Plus 594	AF3715AFP594-100UG
Alexa Fluor Plus 647	AF3715AFP647-100UG
Alexa Fluor Plus 680	AF3715AFP680-100UG
Allophycocyanin	AF3715A
Biotin	BAF3715
CoraFluor 1	AF3715CL1
DyLight 350	AF3715D
DyLight 405	AF3715E
DyLight 488	AF3715K
DyLight 550	AF3715L
DyLight 594	AF3715M
DyLight 650	AF3715W
DyLight 680	AF3715Y
DyLight 755	AF3715Z
FITC	AF3715F
HRP	AF3715H
Janelia Fluor 525	AF3715JF525
Janelia Fluor 549	AF3715I
Janelia Fluor 646	AF3715J
Janelia Fluor 669	AF3715JF669
PE	AF3715P
PerCP	AF3715C
mFluor Violet 450 SE	AF3715MFV450
mFluor Violet 500 SE	AF3715MFV500
mFluor Violet 610 SE	AF3715MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3715-SP

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DPC900

Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

(54 Publications)

1 Kit

DPC900

N/A Fibroblast Activation Protein alpha/FAP [HRP]

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PDPC900
HRP	SPC900

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MAB3715

Fibroblast Activation Protein alpha/FAP Antibody (427819) [Unconjugated]

(25 Publications)

100 μg

MAB3715

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

U-87 MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

The expression of FAP and EGFR and clinical outcome in 132 cases of esophageal cancer. (A) IHC for FAP and EGFR are shown at low and high magnification (40 × and 200 ×). Scale bars: 500 µm (40 ×), 100 µm (200 ×). The expression of FAP and EGFR is different between intra-tumoral and peri-tumoral tissue. (B) Representative example of a low- and high-FAP and EGFR case. Scale bars: 200 µm (100 ×). The FAP score of 4 + or more were defined as high, that of 3 + or less were low; the EGFR expression level of 2 + or 3 + were defined as high, that of 0 or 1 + were low. (C) Survival analysis showed that FAP high patients had significantly worse OS than those with low FAP (P < 0.001, log-rank test; *P < 0.05). (D) Patients with high EGFR tended to show poorer OS than those with low EGFR (P = 0.074, log-rank test). (E) Survival curve of three groups divided by combination of EGFR and FAP scores (double negative, single positive, double positive), double positive group had worse survival than others (P = 0.005: vs. double negative, P = 0.089: vs. single positive, log-rank test; *P < 0.05). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36418422), licensed under a CC-BY license. Not internally tested by R&D Systems.

3715-SE) was serially diluted and captured by Mouse Anti-Human Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB3715) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

3715-SE) was serially diluted and captured by Mouse Anti-Human Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB3715) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3715U-100UG
Alexa Fluor 488	FAB3715G-100
Alexa Fluor 488	FAB3715G-025
Alexa Fluor 532	FAB3715X
Alexa Fluor 594	FAB3715T-025
Alexa Fluor 647	FAB3715R-100UG
Alexa Fluor 700	FAB3715N-100UG
Alexa Fluor 750	FAB3715S-100UG
Alexa Fluor Plus 405	FAB3715AFP405-100UG
Alexa Fluor Plus 488	FAB3715AFP488-100UG
Alexa Fluor Plus 555	FAB3715AFP555-100UG
Alexa Fluor Plus 594	FAB3715AFP594-100UG
Alexa Fluor Plus 647	FAB3715AFP647-100UG
Alexa Fluor Plus 680	FAB3715AFP680-100UG
Allophycocyanin	FAB3715A-100
Allophycocyanin	FAB3715A-025
Allophycocyanin/Cy7	FAB3715APCCY7
Biotin	FAB3715B
CoraFluor 1	FAB3715CL1
DyLight 350	FAB3715D
DyLight 405	FAB3715E
DyLight 488	FAB3715K
DyLight 550	FAB3715L
DyLight 594	FAB3715M
DyLight 650	FAB3715W
DyLight 680	FAB3715Y
DyLight 755	FAB3715Z
FITC	FAB3715F
HRP	FAB3715H
Janelia Fluor 525	FAB3715JF525
Janelia Fluor 549	FAB3715I
Janelia Fluor 646	FAB3715J
Janelia Fluor 669	FAB3715JF669
PE/Atto594	FAB3715PEATT594
PE/Cy5.5	FAB3715PECY55
PE/Cy7	FAB3715PECY7
PerCP	FAB3715C
Phycoerythrin	FAB3715P-100
Phycoerythrin	FAB3715P-025
mFluor Violet 450 SE	FAB3715MFV450
mFluor Violet 500 SE	FAB3715MFV500
mFluor Violet 610 SE	FAB3715MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3715A-025
Carrier Free	FAB3715P-025
Carrier Free	MAB3715-SP
Carrier Free	MAB3715-500
Carrier Free	FAB3715T-025
Carrier Free	FAB3715G-025
Carrier Free	FAB3715U-100UG
Carrier Free	FAB3715S-100UG
Carrier Free	FAB3715R-100UG
Carrier Free	FAB3715N-100UG
Carrier Free	FAB3715G-100
Carrier Free	FAB3715A-100
Carrier Free	FAB3715P-100

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3715-SE

Recombinant Human FAP Protein, CF

(18 Publications)

10 μg

3715-SE

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-14728

Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)

100 ul

NBP3-14728

FAP was detected in immersion fixed paraffin-embedded sections of human Melanoma using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm of fibroblasts. Protocol available in COMET™ Panel Builder.

FAP was detected in immersion fixed paraffin-embedded sections of human Colon Cancer using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm of fibroblasts. Protocol available in COMET™ Panel Builder.

FAP was detected in immersion fixed paraffin-embedded sections of human Pancreas using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of pancreatic islet cells. Protocol available in COMET™ Panel Builder.

Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Whole cell lysate (500 ug per IP reaction; 5% of IPloaded) from IMR-90 cells. Antibody: Rabbit anti-FAPrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blottingimmunoprecipitated FAP, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti FAP recombinant monoclonal antibody [BLR150J] used at 1:1000. Secondary: HRP conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.

ICC/IF- Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB110-85534

Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

(4 Publications)

0.1 mg

NB110-85534

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.

Identification of rAd‐infected DCs in vitro. (A–C) rAd‐FAP, rAd‐hlivin alpha , and rAd‐FAP/hlivin alpha were constructed using mouse FAP cDNA and human livin alpha cDNA, and then transduced into mouse bone marrow‐derived DCs, respectively. For evaluation of transduction efficacy, western blot was performed to measure protein expressions of FAP and livin alpha in cells, with GAPDH functioned as a loading control. ***p < .001, versus 1#; +++p < .001, versus 2#; ^^^p < .001, versus 3#. DCs, dendritic cells; FAP, fibroblast activation protein‐ alpha ; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin alpha , recombinant adenoviral vector encoding human livin alpha cDNA. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37773704), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-85534AF350
Alexa Fluor 405	NB110-85534AF405
Alexa Fluor 488	NB110-85534AF488
Alexa Fluor 532	NB110-85534AF532
Alexa Fluor 594	NB110-85534AF594
Alexa Fluor 647	NB110-85534AF647
Alexa Fluor 700	NB110-85534AF700
Alexa Fluor 750	NB110-85534AF750
Alexa Fluor Plus 405	NB110-85534AFP405
Alexa Fluor Plus 488	NB110-85534AFP488
Alexa Fluor Plus 555	NB110-85534AFP555
Alexa Fluor Plus 594	NB110-85534AFP594
Alexa Fluor Plus 647	NB110-85534AFP647
Alexa Fluor Plus 680	NB110-85534AFP680
Allophycocyanin	NB110-85534APC
Biotin	NB110-85534B
CoraFluor 1	NB110-85534CL1
DyLight 350	NB110-85534UV
DyLight 405	NB110-85534V
DyLight 488	NB110-85534G
DyLight 550	NB110-85534R
DyLight 594	NB110-85534DL594
DyLight 650	NB110-85534C
DyLight 680	NB110-85534FR
DyLight 755	NB110-85534IR
FITC	NB110-85534F
HRP	NB110-85534H
Janelia Fluor 525	NB110-85534JF525
Janelia Fluor 549	NB110-85534JF549
Janelia Fluor 646	NB110-85534JF646
Janelia Fluor 669	NB110-85534JF669
PE	NB110-85534PE
PerCP	NB110-85534PCP
mFluor Violet 450 SE	NB110-85534MFV450
mFluor Violet 500 SE	NB110-85534MFV500
mFluor Violet 610 SE	NB110-85534MFV610

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DY3715

Human FAP DuoSet ELISA, 15 Plate

(11 Publications)

1 Kit (for 15 Plates)

DY3715

N/A Fibroblast Activation Protein alpha/FAP [Biotin]

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB9727

Fibroblast Activation Protein alpha/FAP Antibody (983802) [Unconjugated]

(6 Publications)

100 μg

MAB9727

Fibroblast Activation Protein a/FAP was detected in immersion fixed C2C12 mouse myoblast cell line using Rat Anti-Mouse Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB9727) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Used 3uL for 15mins at 4°C on 3T3 cells transduced to over express murine FAP. Image from a verified customer review.

FAP expression is up-regulated in the myocardium of isoproterenol (ISO) mice and in cardiac fibroblasts upon TGF-beta 1 stimulation. (A) Quantitative polymerase chain reaction (qPCR) analysis showing messenger RNA (mRNA) expression levels of Col1 alpha 1, Acta2, and FAP in heart tissue from ISO-treated mice. n = 6. Data are presented as mean ± SEM. **P < 0.01; ****P < 0.0001. (B) Western blotting analysis of Col1 alpha 1, alpha -smooth muscle actin ( alpha -SMA), and FAP protein expressions in the hearts of mice treated with ISO or saline. (C) Sirius Red staining and immunohistochemical staining for FAP in hearts from ISO-treated mice. (D) mRNA expression levels of Col1 alpha 1, Acta2, and FAP in neonatal rat cardiac fibroblasts (NRCFs) treated with TGF-beta 1 (10 ng/ml) for 24 h, assessed by qPCR. (E) Western blotting analysis of FAP protein expression in NRCFs after TGF-beta 1 treatment. (F) Flow cytometry analysis of FAP protein expression in NRCFs following TGF-beta 1 treatment. (G) Immunofluorescence (IF) analysis of FAP protein expression in NRCFs post-TGF-beta 1 treatment. IHC, immunohistochemical; Col I, type I collagen; PBS, phosphate-buffered saline; Ctrl, control; GAPDH, glyceraldehyde-3-phosphate; DAPI, 4′,6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40225952), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9727U-100UG
Alexa Fluor 405	FAB9727V-100UG
Alexa Fluor 488	FAB9727G-100UG
Alexa Fluor 532	FAB9727X
Alexa Fluor 594	FAB9727T-100UG
Alexa Fluor 647	FAB9727R-100UG
Alexa Fluor 700	FAB9727N-100UG
Alexa Fluor 750	FAB9727S-100UG
Alexa Fluor Plus 405	FAB9727AFP405-100UG
Alexa Fluor Plus 488	FAB9727AFP488-100UG
Alexa Fluor Plus 555	FAB9727AFP555-100UG
Alexa Fluor Plus 594	FAB9727AFP594-100UG
Alexa Fluor Plus 647	FAB9727AFP647-100UG
Alexa Fluor Plus 680	FAB9727AFP680-100UG
Allophycocyanin	FAB9727A
Allophycocyanin/Cy7	FAB9727APCCY7
Biotin	FAB9727B
CoraFluor 1	FAB9727CL1
DyLight 350	FAB9727D
DyLight 405	FAB9727E
DyLight 488	FAB9727K
DyLight 550	FAB9727L
DyLight 594	FAB9727M
DyLight 650	FAB9727W
DyLight 680	FAB9727Y
DyLight 755	FAB9727Z
FITC	FAB9727F
HRP	FAB9727H
Janelia Fluor 525	FAB9727JF525
Janelia Fluor 549	FAB9727I
Janelia Fluor 646	FAB9727J
Janelia Fluor 669	FAB9727JF669
PE	FAB9727P
PE/Atto594	FAB9727PEATT594
PE/Cy5.5	FAB9727PECY55
PE/Cy7	FAB9727PECY7
PerCP	FAB9727C
mFluor Violet 450 SE	FAB9727MFV450
mFluor Violet 500 SE	FAB9727MFV500
mFluor Violet 610 SE	FAB9727MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB9727S-100UG
Carrier Free	FAB9727T-100UG
Carrier Free	FAB9727G-100UG
Carrier Free	FAB9727N-100UG
Carrier Free	FAB9727V-100UG
Carrier Free	FAB9727R-100UG
Carrier Free	FAB9727U-100UG
Carrier Free	MAB9727-SP

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H00002191-M01

Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free

(1 Review)

(6 Publications)

0.1 mg

H00002191-M01

Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP transfected lysate using anti-FAP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAP MaxPab rabbit polyclonal antibody.

ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Detection limit for recombinant GST tagged FAP is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-28536

Fibroblast Activation Protein alpha/FAP Antibody - Humanized, (Research Grade sibrotuzumab Biosimilar) - Low Endotoxin, Azide and BSA Free

100 μg

NBP3-28536

Immobilized human FAP His at 2 ug/mL can bind Fibroblast Activation Protein alpha/FAP Antibody (sibrotuzumab) - Humanized, EC50=0.00419 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28536AF350
Alexa Fluor 405	NBP3-28536AF405
Alexa Fluor 488	NBP3-28536AF488
Alexa Fluor 532	NBP3-28536AF532
Alexa Fluor 594	NBP3-28536AF594
Alexa Fluor 647	NBP3-28536AF647
Alexa Fluor 700	NBP3-28536AF700
Alexa Fluor 750	NBP3-28536AF750
Alexa Fluor Plus 405	NBP3-28536AFP405
Alexa Fluor Plus 488	NBP3-28536AFP488
Alexa Fluor Plus 555	NBP3-28536AFP555
Alexa Fluor Plus 594	NBP3-28536AFP594
Alexa Fluor Plus 647	NBP3-28536AFP647
Alexa Fluor Plus 680	NBP3-28536AFP680
Allophycocyanin	NBP3-28536APC
Biotin	NBP3-28536B
CoraFluor 1	NBP3-28536CL1
DyLight 350	NBP3-28536UV
DyLight 405	NBP3-28536V
DyLight 488	NBP3-28536G
DyLight 550	NBP3-28536R
DyLight 594	NBP3-28536DL594
DyLight 650	NBP3-28536C
DyLight 680	NBP3-28536FR
DyLight 755	NBP3-28536IR
FITC	NBP3-28536F
HRP	NBP3-28536H
Janelia Fluor 525	NBP3-28536JF525
Janelia Fluor 549	NBP3-28536JF549
Janelia Fluor 646	NBP3-28536JF646
Janelia Fluor 669	NBP3-28536JF669
PE	NBP3-28536PE
PerCP	NBP3-28536PCP
mFluor Violet 450 SE	NBP3-28536MFV450
mFluor Violet 500 SE	NBP3-28536MFV500
mFluor Violet 610 SE	NBP3-28536MFV610

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NBP2-66844

Fibroblast Activation Protein alpha/FAP Antibody (JA56-11)

(1 Publication)

100 ul

NBP2-66844

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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8647-SE

Recombinant Mouse FAP Protein, CF

(2 Publications)

10 μg

8647-SE

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

FAP Products By Gene Name

Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

Fibroblast Activation Protein alpha/FAP Antibody (427819) [Unconjugated]

Recombinant Human FAP Protein, CF

Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)

Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Human FAP DuoSet ELISA, 15 Plate

Fibroblast Activation Protein alpha/FAP Antibody (983802) [Unconjugated]

Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free

Fibroblast Activation Protein alpha/FAP Antibody - Humanized, (Research Grade sibrotuzumab Biosimilar) - Low Endotoxin, Azide and BSA Free

Fibroblast Activation Protein alpha/FAP Antibody (JA56-11)

Recombinant Mouse FAP Protein, CF