Fibroblast Activation Protein alpha/FAP Products

HAF016). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">

HAF016). A specific band was detected for Fibroblast Activation Protein  alpha /FAP at approximately 97 kDa (as indicated) in the parental WI‑38 human lung fibroblast cell line, but is not detectable in knockout WI‑38 human lung fibroblast cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">

AF3715) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. " class="big_lightbox" target="_blank">

3715-SE) was serially diluted and captured by Mouse Anti-Human Fibroblast Activation Protein  alpha /FAP Monoclonal Antibody (Catalog # MAB3715) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Fibroblast Activation Protein  alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">

MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox" target="_blank">

3715-SE) was serially diluted and captured by Mouse Anti-Human Fibroblast Activation Protein  alpha /FAP Monoclonal Antibody (Catalog # MAB3715) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Fibroblast Activation Protein  alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">