Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Fibroblast Activation Protein a/FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of IMR‑90 human lung fibroblast cell line and WI‑38 human lung fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 130 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of WI‑38 human lung fibroblast cell line and human FAP knockout WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 97 kDa (as indicated) in the parental WI‑38 human lung fibroblast cell line, but is not detectable in knockout WI‑38 human lung fibroblast cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formalin-fixed paraffin-embedded tissue sections of human squamous cell carcinoma in the esophagus were probed for FAP mRNA (ACD RNAScope Probe, catalog # 411971; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using sheep anti-human FAP polyclonal antibody (R&D Systems catalog # AF3715) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to connective tissue.

FGFR2 drives the process of liver fibrosis. (A) The expression of liver fibrosis markers (ACTA2, fibroblast activation protein alpha (FAP), alpha-1 type I collagen (COL1A1)) was measured by qPCR. The samples were divided into four groups according to whether cells were induced with or without TGF-beta and whether cells were overexpressed by FGFR2. (B) The expression of markers in wild-type and FGFR2-OE cell lines under TGF-beta induction was analyzed by Western blot analysis. (C) The expression levels of alpha -SMA and (D) collagen secretion in LX-2 and Huh-7 were evaluated, when FGFR2 was overexpressed or knocked down upon equal TGF-beta induction. (E) Cells were collected after 48 h of co-culture, and the expression of alpha -SMA and (F) type I collagen in the lower layer cells was determined by ELISA. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

CYN blocks the activation and development of liver fibrosis in vitro. (A) Evaluation of the inhibitory effect of CYN on the fibrosis-promoting effect of FGFR2. The fibrotic transformation of wild-type and FGFR2-OE LX-2 cells and Huh-7 cells was induced through TGF-beta activation, followed by an intervention with CYN for the relevant groups. The expression changes of the fibrosis markers ACTA2 and COL1A1 were analyzed by qPCR. (B) Wild-type cell lines were employed in the aforementioned experiments, and the activation of FGFR2 was triggered by supplementation with the exogenous basic fibroblast growth factor (bFGF) factor. (C) Analysis of the extent of antagonism of CYN towards TGF-beta Signaling. Activation induction models were established by adding or not adding TGF-beta to the cell culture environment with or without the CYN intervention, and the expression of liver fibrosis markers was determined by qPCR, (D) Western blot, and (E,F) ELISA analyses. (G) A co-culture model was used to evaluate the blocking effect of CYN on liver fibrosis activation of signaling transmission. The activation intensity of lower-layer wild-type cells was measured and compared using alpha -SMA expression and collagen secretion. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

CYN blocks the activation and development of liver fibrosis in vitro. (A) Evaluation of the inhibitory effect of CYN on the fibrosis-promoting effect of FGFR2. The fibrotic transformation of wild-type and FGFR2-OE LX-2 cells and Huh-7 cells was induced through TGF-beta activation, followed by an intervention with CYN for the relevant groups. The expression changes of the fibrosis markers ACTA2 and COL1A1 were analyzed by qPCR. (B) Wild-type cell lines were employed in the aforementioned experiments, and the activation of FGFR2 was triggered by supplementation with the exogenous basic fibroblast growth factor (bFGF) factor. (C) Analysis of the extent of antagonism of CYN towards TGF-beta Signaling. Activation induction models were established by adding or not adding TGF-beta to the cell culture environment with or without the CYN intervention, and the expression of liver fibrosis markers was determined by qPCR, (D) Western blot, and (E,F) ELISA analyses. (G) A co-culture model was used to evaluate the blocking effect of CYN on liver fibrosis activation of signaling transmission. The activation intensity of lower-layer wild-type cells was measured and compared using alpha -SMA expression and collagen secretion. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

High expression of fibroblast growth factor receptor 2 (FGFR2) coincides with liver fibrosis. (A) The expression of FGFR2 in fibrotic liver and normal liver tissues was evaluated through data mining of human samples affected by Hepatitis B infection (GSE38941), alcohol abuse (GSE28619), and nonalcoholic steatohepatitis (GSE48452), as well as mouse samples induced with carbon tetrachloride (CCl4) (GSE152329, GSE98577). The differential fold expression was determined by comparing the expression of FGFR2 in the fibrotic liver and normal liver tissues. (B) A comparison of changes in FGFR2 expression in liver fibrosis patients in remission (improved) or not in remission (not improved) with mining data of GSE175448. (C) The correlation between FGFR2 expression and the liver fibrosis score (Ishak score) was analyzed by comparing FGFR2 expression in three pairs of liver fibrotic tissues and normal liver tissues through immunohistochemistry. (D) FGFR2 expression in liver fibrosis tissues and normal liver tissues was verified by Western blot and (E) q-PCR analyses. (E) Liver tissues from mice treated with CCl4 for different durations were used to determine the expression of the liver fibrosis markers Actin Alpha 2 (ACTA2) and FGFR2 by q-PCR and the trends of their expression with increasing days of induction. (F) The expression of ACTA2 and (G) FGFR2 was determined by q-PCR in liver tissues from mice treated with CCl4 for varying durations. The statistical significance was determined based on the p-value. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, “***” when p < 0.001, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

High expression of fibroblast growth factor receptor 2 (FGFR2) coincides with liver fibrosis. (A) The expression of FGFR2 in fibrotic liver and normal liver tissues was evaluated through data mining of human samples affected by Hepatitis B infection (GSE38941), alcohol abuse (GSE28619), and nonalcoholic steatohepatitis (GSE48452), as well as mouse samples induced with carbon tetrachloride (CCl4) (GSE152329, GSE98577). The differential fold expression was determined by comparing the expression of FGFR2 in the fibrotic liver and normal liver tissues. (B) A comparison of changes in FGFR2 expression in liver fibrosis patients in remission (improved) or not in remission (not improved) with mining data of GSE175448. (C) The correlation between FGFR2 expression and the liver fibrosis score (Ishak score) was analyzed by comparing FGFR2 expression in three pairs of liver fibrotic tissues and normal liver tissues through immunohistochemistry. (D) FGFR2 expression in liver fibrosis tissues and normal liver tissues was verified by Western blot and (E) q-PCR analyses. (E) Liver tissues from mice treated with CCl4 for different durations were used to determine the expression of the liver fibrosis markers Actin Alpha 2 (ACTA2) and FGFR2 by q-PCR and the trends of their expression with increasing days of induction. (F) The expression of ACTA2 and (G) FGFR2 was determined by q-PCR in liver tissues from mice treated with CCl4 for varying durations. The statistical significance was determined based on the p-value. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, “***” when p < 0.001, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression analysis of Fap in OA synovium. a Immunostaining of human FAP in the synovium of control and OA patients. DAPI staining indicates the nucleus. Scale bars: 100 μm. b qPCR analysis of human FAP mRNA levels in the synovium of control and OA patients (n = 8 samples per group). c Western blot analysis of human FAP protein levels in the synovium of control and OA patients (n = 3 samples per group). d Immunostaining of mouse Fap in the knee joint of sham and DMM-treated mice. Sham or DMM surgery was performed in 8-week-old FapLacZ/+ mice, which were sacrificed 8 weeks later, and LacZ immunostaining was used to detect Fap expression at the posterior horn of the medial meniscus (F: femur; T: tibia; M: meniscus; S: synovium). DAPI staining indicates the nucleus. Scale bars: 100 μm. The statistical significance was assessed using two-tailed Student’s unpaired t tests. Data are presented as the mean ± SD (**P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36588124), licensed under a CC-BY license. Not internally tested by R&D Systems.

FGFR2 drives the process of liver fibrosis. (A) The expression of liver fibrosis markers (ACTA2, fibroblast activation protein alpha (FAP), alpha-1 type I collagen (COL1A1)) was measured by qPCR. The samples were divided into four groups according to whether cells were induced with or without TGF-beta and whether cells were overexpressed by FGFR2. (B) The expression of markers in wild-type and FGFR2-OE cell lines under TGF-beta induction was analyzed by Western blot analysis. (C) The expression levels of alpha -SMA and (D) collagen secretion in LX-2 and Huh-7 were evaluated, when FGFR2 was overexpressed or knocked down upon equal TGF-beta induction. (E) Cells were collected after 48 h of co-culture, and the expression of alpha -SMA and (F) type I collagen in the lower layer cells was determined by ELISA. The results are marked as significant “*” when p < 0.05, “**” when p < 0.01, and not significant (ns) if p ≥ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37111305), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression analysis of Fap in OA synovium. a Immunostaining of human FAP in the synovium of control and OA patients. DAPI staining indicates the nucleus. Scale bars: 100 μm. b qPCR analysis of human FAP mRNA levels in the synovium of control and OA patients (n = 8 samples per group). c Western blot analysis of human FAP protein levels in the synovium of control and OA patients (n = 3 samples per group). d Immunostaining of mouse Fap in the knee joint of sham and DMM-treated mice. Sham or DMM surgery was performed in 8-week-old FapLacZ/+ mice, which were sacrificed 8 weeks later, and LacZ immunostaining was used to detect Fap expression at the posterior horn of the medial meniscus (F: femur; T: tibia; M: meniscus; S: synovium). DAPI staining indicates the nucleus. Scale bars: 100 μm. The statistical significance was assessed using two-tailed Student’s unpaired t tests. Data are presented as the mean ± SD (**P < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36588124), licensed under a CC-BY license. Not internally tested by R&D Systems.

AREG promotes migration and CAF-like differentiation of MSCs: (A,B) To investigate the differentiation of MSCs into CAFs upon treatment with rhAREG (100 ng/mL), the mRNA and protein expression levels of FAP, IL-6, and alpha SMA were compared using qRT-PCR (A) and Western blotting (B). beta -actin was used as a loading control (B). (C,D) The effects of rhAREG (100 ng/mL) on the survival (C) and growth (D) of MSCs were evaluated using the MTS assay. (E) The effect of rhAREG (100 ng/mL) on the migration of MSCs was evaluated using the transwell migration assay. MSCs were seeded in the upper chamber, and migrated cells were counted in five fields of view after 48 h. Representative images for each condition are presented below the graph (E). The data are presented as the mean ± SEM of three independent experiments (A,C–E). N.S., not significant; ** p < 0.01, *** p < 0.001. Scale bars: 100 μm (E). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39451251), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, IP, Dual ISH-IHC, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
  Leu26-Asp760
  Accession # Q12884
• Specificity: Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FAP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated 0.5 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.

1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
4. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
5. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Activation Protein alpha/FAP Antibody (AF3715)(51)

Publications using AF3715

• Yokoo, H;Koma, YI;Nomura, N;Torigoe, R;Omori, M;Nakanishi, T;Miyako, S;Nakanishi, T;Kodama, T;Shigeoka, M;Kakeji, Y;Horie, M; BGN Secreted by Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression via Activation of TLR4-Mediated Erk and NF-?B Signaling Pathways International journal of molecular sciences 2025-12-13 [PMID: 41465449] (Western Blot, Human)
• Lee, JY;Reyes, N;Woo, SH;Allen, NC;Kadota, T;Lechner, A;Biswas, R;Goel, S;Stratton, F;Kuang, C;Tsukui, T;Auyeung, V;Mansfield, AS;LaFave, LM;Peng, T; Senolytic-sensitive p16Ink4a+ fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity Cell stem cell 2025-11-03 [PMID: 41187746] (Immunohistochemistry, Human)
• Chen, W;Jiang, M;Zou, X;Chen, Z;Shen, L;Hu, J;Kong, M;Huang, J;Ni, C;Xia, W; Fibroblast Activation Protein (FAP)+ cancer-associated fibroblasts induce macrophage M2-like polarization via the Fibronectin 1-Integrin ?5?1 axis in breast cancer Oncogene 2025-04-22 [PMID: 40263422] (Immunohistochemistry, Human)
• Kang, JY;Mun, D;Park, M;Yoo, G;Kim, H;Yun, N;Joung, B; Injured Cardiac Tissue-Targeted Delivery of TGF?1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair International journal of nanomedicine 2025-03-01 [PMID: 40046817] (Immunohistochemistry, Western Blot, Human, Mouse)
• Di Carlo, E;Sorrentino, C; The multifaceted role of the stroma in the healthy prostate and prostate cancer Journal of translational medicine 2024-09-05 [PMID: 39238004] (Immunohistochemistry, Human)
• Lee, JY;Reyes, N;Woo, SH;Goel, S;Stratton, F;Kuang, C;Mansfield, AS;LaFave, LM;Peng, T; Senescent fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity bioRxiv : the preprint server for biology 2024-07-30 [PMID: 39131266] (Immunohistochemistry, Transgenic Mouse)
• Oguri, N;Gi, T;Nakamura, E;Furukoji, E;Goto, H;Maekawa, K;Tsuji, AB;Nishii, R;Aman, M;Moriguchi-Goto, S;Sakae, T;Azuma, M;Yamashita, A; Expression of fibroblast activation protein-? in human deep vein thrombosis Thrombosis research 2024-06-25 [PMID: 38955058] (Immunocytochemistry, Human)
• Susanne N. Wijesinghe, Amel Badoume, Dominika E. Nanus, Archana Sharma‐Oates, Hussein Farah, Michelangelo Certo et al. Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets Clinical and Translational Medicine 2023-04-03 [PMID: 37006170]
• Noriko Mori, Jiefu Jin, Balaji Krishnamachary, Yelena Mironchik, Flonné Wildes, Farhad Vesuna et al. Functional roles of FAP-alpha in metabolism, migration and invasion of human cancer cells Frontiers in Oncology 2023-03-01 [PMID: 36937451]
• A Pappalardo, D Alvarez Ce, S Fang, AR Herschman, EY Jeon, KM Myers, JW Kysar, HE Abaci Engineering edgeless human skin with enhanced biomechanical properties Science Advances, 2023-01-27;9(4):eade2514. 2023-01-27 [PMID: 36706190] (IHC, Human)
• Tania Baltazar, Bo Jiang, Alejandra Moncayo, Jonathan Merola, Mohammad Z. Albanna, W. Mark Saltzman et al. 3D bioprinting of an implantable xeno‐free vascularized human skin graft Bioengineering & Translational Medicine 2023-01-01 [PMID: 36684084]
• Aoyuan Fan, Genbin Wu, Jianfang Wang, Laiya Lu, Jingyi Wang, Hanjing Wei et al. Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression Bone Research 2023-01-02 [PMID: 36588124]
• B Li, Z Ding, O Calbay, Y Li, T Li, L Jin, S Huang FAP is critical for ovarian cancer cell survival by sustaining NF-kappaB activation through recruitment of PRKDC in lipid rafts Cancer Gene Therapy, 2022-12-09;0(0):. 2022-12-09 [PMID: 36494579] (IP, Mouse)
• W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Flow Cytometry, IHC, Western Blot, Human)
• Jiefu Jin, James D. Barnett, Balaji Krishnamachary, Yelena Mironchik, Catherine K. Luo, Hisataka Kobayashi et al. Evaluating near‐infrared photoimmunotherapy for targeting fibroblast activation protein‐ alpha expressing cells in vitro and in vivo Cancer Science 2023-01-01 [PMID: 36169301]
• RG Marangoni, P Datta, A Paine, S Duemmel, MA Nuzzo, L Sherwood, J Varga, C Ritchlin, BD Korman Thy-1 plays a pathogenic role and is a potential biomarker for skin fibrosis in scleroderma JCI Insight, 2022-10-10;0(0):. 2022-10-10 [PMID: 36066980] (IHC, Mouse)
• M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker Cancers, 2022-07-13;14(14):. 2022-07-13 [PMID: 35884455] (IHC, Human)
• Lingling Ou, Huaishan Wang, Hui Huang, Zhiyan Zhou, Qiang Lin, Yeye Guo et al. Preclinical platforms to study therapeutic efficacy of human gamma δ T cells Clinical and Translational Medicine 6/22/2022 [PMID: 35731974]
• Lingling Ou, Huaishan Wang, Hui Huang, Zhiyan Zhou, Qiang Lin, Yeye Guo et al. Preclinical platforms to study therapeutic efficacy of human gamma δ T cells Clinical and Translational Medicine 2022-06-22 [PMID: 35731974] (Flow Cytometry, Human)
• II Verginadis, H Avgousti, J Monslow, G Skoufos, F Chinga, K Kim, NM Leli, IV Karagounis, BI Bell, A Velalopoul, CS Salinas, VS Wu, Y Li, J Ye, DA Scott, AL Osterman, A Sengupta, A Weljie, M Huang, D Zhang, Y Fan, E Radaelli, JW Tobias, F Rambow, P Karras, JC Marine, X Xu, AG Hatzigeorg, S Ryeom, JA Diehl, SY Fuchs, E Puré, C Koumenis A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression Nature Cell Biology, 2022-06-02;0(0):. 2022-06-02 [PMID: 35654839] (IHC, Mouse)
• Geni Ye, Maohua Huang, Yong Li, Jie Ouyang, Minfeng Chen, Qing Wen et al. The FAP alpha -activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway Acta Pharmaceutica Sinica B 2022-03-01 [PMID: 35530139]
• Laura De Vargas Roditi, Andrea Jacobs, Jan H. Rueschoff, Pete Bankhead, Stéphane Chevrier, Hartland W. Jackson et al. Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer Cell Reports Medicine 2022-04-19 [PMID: 35492239]
• Layla Mathieson, Richard A. O’Connor, Hazel Stewart, Paige Shaw, Kevin Dhaliwal, Gareth O. S. Williams et al. Fibroblast Activation Protein Specific Optical Imaging in Non-Small Cell Lung Cancer Frontiers in Oncology 2022-03-10 [PMID: 35359378] (Flow Cytometry, Human)
• Chivu-Economescu M, Necula LG, Matei L et al. Collagen Family and Other Matrix Remodeling Proteins Identified by Bioinformatics Analysis as Hub Genes Involved in Gastric Cancer Progression and Prognosis International journal of molecular sciences 3/16/2022 [PMID: 35328635]
• JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression Science Advances, 2021-11-03;7(45):eabg9518. 11/3/2021 [PMID: 34730997]
• M Friedrich, M Pohin, MA Jackson, I Korsunsky, SJ Bullers, K Rue-Albrec, Z Christofor, D Sathananth, T Thomas, R Ravindran, R Tandon, RS Peres, H Sharpe, K Wei, GFM Watts, EH Mann, A Geremia, M Attar, Oxford IBD, Roche Fibr, S McCuaig, L Thomas, E Collantes, HH Uhlig, SN Sansom, A Easton, S Raychaudhu, SP Travis, FM Powrie IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies Nature Medicine, 2021-10-21;0(0):. 2021-10-21 [PMID: 34675383] (IHC, Human)
• M Shimizu, YI Koma, H Sakamoto, S Tsukamoto, Y Kitamura, S Urakami, K Tanigawa, T Kodama, N Higashino, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression Cancers, 2021-09-10;13(18):. 2021-09-10 [PMID: 34572779] (Western Blot, Human)
• Richard A O’Connor, Vishwani Chauhan, Layla Mathieson, Helen Titmarsh, Lilian Koppensteiner, Irene Young et al. T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer OncoImmunology 2021-07-22 [PMID: 34290905]
• N Ganig, F Baenke, ML Thepkayson, K Lin, VS Rao, FC Wong, H Polster, M Schneider, D Helm, M Pecqueux, AM Seifert, L Seifert, J Weitz, NN Rahbari, C Kahlert Proteomic Analyses of Fibroblast- and Serum-Derived Exosomes Identify QSOX1 as a Marker for Non-invasive Detection of Colorectal Cancer Cancers, 2021-03-17;13(6):. 2021-03-17 [PMID: 33802764] (IHC, Western Blot, Human)
• LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape J Am Heart Assoc, 2020-11-16;9(23):e017094. 2020-11-16 [PMID: 33190596] (ICC, IHC, Human)
• C Cho, R Mukherjee, AR Peck, Y Sun, N McBrearty, KV Katlinski, J Gui, PK Govindaraj, E Puré, H Rui, SY Fuchs Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis Oncogene, 2020-08-17;0(0):. 2020-08-17 [PMID: 32807917] (ICC, IHC, Human)
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Reviews for Fibroblast Activation Protein alpha/FAP Antibody (AF3715) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Mohamad Youness ICC Human 08/01/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: primary human cardiac fibroblasts
• Species: Human
• Lot: ZKW0622011

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Santhosh Sivajothi IHC-P Human 04/15/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast tumor
• Species: Human
• Lot: ZKW0618101

Bioinformatics

• Gene Symbol: FAP
• Uniprot:
  • Q12884()