FGL2/Fibroleukin Antibody (532223) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 532223
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

FGL2/Fibroleukin Antibody (532223) [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human FGL2
  Asn24-Pro439
  Accession # Q14314
• Specificity: Detects human FGL2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-like 2, and recombinant mouse Angiopoietin-3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGL2/Fibroleukin Antibody (532223) [Alexa Fluor® 350]

• FGL2
• fibrinogen-like 2
• Fibrinogen-like protein 2
• Fibroleukin
• pT49T49
• T49

Background

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64‑70 kDa secreted glycoprotein of the fibrinogen-like superfamily. It is produced by macrophages and endothelial cells in response to inflammatory mediators, T cells and fetal trophoblast cells. FGL2 has prothrombinase activity, promotes immune-mediated thrombosis and functions in transplant rejection and infection-related abortion. Mature human FGL2 gene is a 416 amino acid (aa) protein with a coiled-coil region and a fibronectin C-terminal homology domain or FRED. Coiled-coil dimers are covalently linked to form a tetrameric 260‑280 aa FGL2 complex. Mature human FGL2 shares 79% aa identity with mouse FGL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.