FGFR5/FGFRL1 Antibody (223003) [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clone: 223003
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGFR5/FGFRL1 Antibody (223003) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse FGF R5/FGFRL1
  Ala21-Pro374
  Accession # Q91V87
• Specificity: Detects mouse FGF R5/FGFRL1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF R1 alpha (IIIb), 1 alpha (IIIc), 1 beta (IIIb), 1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), 2 alpha (IIIc), 2 beta (IIIb), 2 beta (IIIc), 3 alpha (IIIb), 3 alpha (IIIc), rmFGF R4, or rhFGF R5 is observed. Was not tested for cross-reactivity with rmFGF R5 gamma .
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Fgfrl1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR5/FGFRL1 Antibody (223003) [Unconjugated]

• FGF homologous factor receptor
• FGF R5
• FGF receptor-like protein 1
• FGFR5
• FGFR-5
• FGFRL1
• FGFR-like protein
• FHFR
• Fibroblast growth factor receptor 5
• fibroblast growth factor receptor-like 1

Background

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2‑6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
2. Sleeman, M. et al. (2001) Gene 271:171.
3. Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
4. Antoine, M. et al. (2005) Growth Factors 23:87.
5. Wiedemann, M. et al. (2000) Genomics 69:275.
6. Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Fgfrl1
• Uniprot:
  • Q91V87()