FGFR3 Antibody (JM81-10)

Western Blot: FGFR3 Antibody (JM81-10) [NBP2-66839] - Analysis of FGFR3 on Hela cells lysates using anti-FGFR3 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM81-10) [NBP2-66839] - Staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM81-10) [NBP2-66839] - Analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Flow Cytometry: FGFR3 Antibody (JM81-10) [NBP2-66839] - FGF R3 Antibody (JM81-10) [NBP2-66839] - Analysis of HepG2 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM81-10) [NBP2-66839] - Staining FGFR3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM81-10) [NBP2-66839] - Staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM81-10) [NBP2-66839] - Analysis of paraffin-embedded human breast cancer tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.

Product Discontinued

FGFR3 Antibody (JM81-10) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human FGFR3 aa 719-806 / 806. (SwissProt: P22607 Human)
• Localization: Endoplasmic reticulum, Cell membrane, Cytoplasmic vesicle, Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FGFR3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FGFR3 Antibody (JM81-10)

• CD333
• CEK
• EC 2.7.10
• FGF R3
• FGFR3
• fibroblast growth factor receptor 3
• HSFGFR3EX
• JTK4
• JTK4thanatophoric dwarfism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGFR3