FGFR3 Antibody (136315) - (IIIb)

Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232‑FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND50 is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL).

• Reactivity: Hu
• Applications: B/N
• Clone: 136315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FGFR3 Antibody (136315) - (IIIb) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIb and IIIc)
• Specificity: Detects human FGF R3 (IIIb) in direct ELISAs. In direct ELISAs, approximately 25‑50% cross-reactivity with recombinant mouse FGF R3 (IIIb) is observed and 12% with recombinant human (rh) FGF R4 is observed and no cross-reactivity with (IIIc) isoforms of rhFGF R3 or any isoforms of rhFGF R1 or rhFGF R2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR3
• Purity: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-2 ug/mL
• Control:

  FGFR3 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136315) - (IIIb)

• CD333
• CEK
• EC 2.7.10
• FGF R3
• FGFR3
• fibroblast growth factor receptor 3
• HSFGFR3EX
• JTK4
• JTK4thanatophoric dwarfism

Background

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfateproteoglycans as cofactors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9,and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR3 Antibody (MAB1474)(1)

Publication using MAB1474

• Tomlinson DC, L&amp;amp;apos;H&amp;amp;#xF4;te CG, Kennedy W, Pitt E, Knowles MA Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines. Cancer Res., 2005;65(22):10441-9. 2005 [PMID: 16288035] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: FGFR3