Recombinant Human FGF18 Protein

Product Discontinued

Recombinant Human FGF18 Protein Summary

• Description: A biologically active protein corresponding to FGF18.
  
  

  Source: E. coli

• Details of Functionality: FGF18 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF18
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 1EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 21.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Concentration: LYOPH
• Purity: >95% pure by SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Alternate Names for Recombinant Human FGF18 Protein

• FGF18
• FGF-18
• FGF-18ZFGF5
• fibroblast growth factor 18
• zFGF5

Background

Human FGF-18 is encoded by the FGF18 gene. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18-/- mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. In addition, it also has significant roles in lung development and has an anabolic effect on cartilage formation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FGF18