FGF-22 Overexpression Lysate

Images

 
Western Blot: FGF-22 Overexpression Lysate (Adult Normal) [NBP2-06629] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF-22.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FGF-22 Overexpression Lysate Summary

Description

FGF-22 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC215757

Accession#: NM_020637

Protein Tag: C-MYC/DDK
Gene
FGF22

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FGF-22 Overexpression Lysate

  • FGF22
  • FGF-22
  • fibroblast growth factor 22

Background

Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. Fibroblast growth factor- 22 (FGF-22) is a secreted protein whose gene is localized to human chromosome 19p13.3. FGF-22 is expressed preferentially in the inner root sheath of the hair follicle, suggesting that it is important in hair development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol FGF22