Recombinant Human FGF-12 Protein

Product Discontinued

Recombinant Human FGF-12 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-181 of Human FGF-12
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF12
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris buffer (pH 7.5), 10% glycerol, 1 mM DTT, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 50 ul of distilled water to a final concentration is 1.0 mg/ml.

Alternate Names for Recombinant Human FGF-12 Protein

• FGF12
• FGF-12
• FGF12B
• FHF1
• FHF-1
• FHF1Fibroblast growth factor homologous factor 1
• fibroblast growth factor 12
• fibroblast growth factor 12B
• fibroblast growth factor FGF-12b
• Myocyte-activating factor

Background

FGF12 (Fibroblast growth factor 12) is a member of the FGF superfamily of molecules which currently stands at 22 members. The FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF12 binds to IB2 (islet brain-2), a cellular kinase scaffold, and voltage gated sodium channels and also plays an important role in intracellular signaling and ion exchange. Recombinant human FGF12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FGF12