FGD4 Overexpression Lysate (Native)

Images

 
Western Blot: FGD4 Overexpression Lysate (Adult Normal) [NBL1-10686] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGD4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FGD4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219442 and based on accession number NM_139241. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens FYVE, RhoGEF and PH domain containing 4 (FGD4), mRNA.
Gene
FGD4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
86.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FGD4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FGD4 Overexpression Lysate (Native)

  • Actin filament-binding protein frabin
  • CMT4HFLJ42663
  • DKFZp313E1818
  • FGD1 family, member 4
  • frabin
  • FRABPDKFZp434K1572
  • FYVE, RhoGEF and PH domain containing 4
  • MGC57222
  • RhoGEF and PH domain-containing protein 4
  • ZFYVE6FLJ34370

Background

This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FGD4