FFAR3/GPR41 Antibody (2590G) [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human FFAR3/GPR41 Monoclonal Antibody (Catalog # MAB10562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for FFAR3/GPR41 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

U937 human histiocytic lymphoma cell line was stained with Rabbit anti-human FFAR3/GPR41 monoclonal antibody (Catalog # MAB10562, open histogram) or isotype control antibody (MAB1050, open histogram) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 2590G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

FFAR3/GPR41 Antibody (2590G) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing human FFAR3
• Specificity: Detects human FFAR3 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FFAR3/GPR41 Antibody (2590G) [Unconjugated]

• FFA3
• FFA3R
• FFAR3
• free fatty acid receptor 3
• G protein-coupled receptor 41
• GPR41
• GPR41FFA3R
• G-protein coupled receptor 41

Background

GPR41 and GPR42 are two closely related genes that are part of a cluster of four adjacent G protein-coupled receptors(GPR40, 41, 42, and 43). There are only six nucleotide and amino acid differences between GPR41 and GPR42. GPR41 is a receptor for short chain fatty acids. The rank order of potency for agonists of this receptor ispropionate = pentanoate= butyrate > acetate > formate. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.