FERMT3/URP2 Antibody

Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human FERMT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7004) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FERMT3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

FERMT3/URP2 Antibody Summary

• Immunogen: E. coli-derived recombinant human FERMT3
  Ala498-Phe667
  Accession # Q86UX7
• Specificity: Detects human FERMT3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) FERMT2 is observed and less than 1% cross-reactivity with rhFERMT1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FERMT3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FERMT3/URP2 Antibody

• fermitin family member 3
• FERMT3
• KIND3
• KIND3fermitin family homolog 3 (Drosophila)
• Kindlin 3
• Kindlin-3
• MGC10966
• MIG-2
• MIG2B
• MIG2BURP2SF
• MIG2-like protein
• UNC-112 related protein 2
• UNC112C
• Unc-112-related protein 2
• URP2
• URP2fermitin family homolog 3
• URP2SF

Background

 

FERMT3 (Fermitin family homolog 3; also kindling-3, MIG2B and URP2) is a 72-78 kDa member of the kindling family of proteins. It is a cytoplasmic protein that is expressed by B cells, T cells, platelets, neutrophils and vascular endothelial cells. FERMT3 acts on the cytoplasmic tails of beta -integrins 1 thru 3 to induce a conformational change and activation. This allows for expressing cell adhesion and arrest. Human FERMT3 is 667 amino acids (aa) in length. It contains a poly-Lys region (aa 147-155) plus a FERM domain (aa 229-558) that itself possesses a membrane-anchoring Pleckstrin homology domain (aa 354-457). There are three utilized phosphorylation sites at Ser8, Tyr11 and Tyr504. There are also two splice variants. One is 56 kDa in size and represents the use of an alternative start site at Met181, while a second contains a deletion of aa 360-363. Over aa 498-667, human FERMT3 shares 99% aa identity with mouse FERMT3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FERMT3
• Uniprot:
  • Q86UX7(Human)