FCRLB/FCRY Antibody (465905)

• Reactivity: Mu
• Applications: WB
• Clone: 465905
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

FCRLB/FCRY Antibody (465905) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse FCRLB/FCRY
  Ala18-Ser427
  Accession # Q5DRQ8
• Specificity: Detects mouse FCRLB/FCRY in direct ELISAs and Western blots. In Western blots, 75% cross-reactivity with recombinant human FCRLB is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FCRLB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRLB/FCRY Antibody (465905)

• Fc receptor homolog expressed in B cells protein 2
• Fc receptor like 2
• Fc receptor-like and mucin-like 2
• Fc receptor-like and mucin-like protein 2
• Fc receptor-like B
• Fc receptor-like protein 2
• Fc receptor-related protein Y
• FcRL2
• FCRL2FCRLM2
• FCRLB
• FCRLM2
• FCRLY
• FCRY
• FLJ31052
• FREB2
• FREB-2
• FREB-2FcRY
• RP11-474I16.6

Background

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82% and 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1‑3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1‑4). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is upregulated in B cells by BAFF and LPS (3). Among non‑hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (3, 5).

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
3. Masuda, K. et al. (2005) Gene 363:32.
4. Chikaev, N.A. et al. (2005) Genomics 85:264.
5. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
6. Inozume, T. et al. (2005) Int. J. Cancer 114:283.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCRLB
• Uniprot:
  • Q5DRQ8()