FCRL1/FcRH1 Antibody (282415) [Unconjugated]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 282415
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FCRL1/FcRH1 Antibody (282415) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FCRL1/FcRH1
  Ala17-His304
  Accession # Q96LA6
• Specificity: Detects human FCRL1/FcRH1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FCRL2, 3, 4, or 5 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCRL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRL1/FcRH1 Antibody (282415) [Unconjugated]

• BXMH1
• CD307a antigen
• CD307a
• Fc receptor homolog 1
• Fc receptor-like 1
• FcRH1
• FCRH1DKFZp667O1421
• FCRL1
• FcR-like protein 1
• hIFGP1
• IFGP family protein 1
• IFGP1
• IFGP1Fc receptor-like protein 1
• Immune receptor translocation-associated protein 5
• IRTA5
• IRTA5immunoglobulin superfamily Fc receptor, gp42

Background

Fc Receptor-Like 1 (FCRL1), also known as FcRH1, and IRTA5, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified human IgG. Mature human FCRL1 consists of a 291 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain with two immunotyrosine activation motifs (ITIMs) (3, 4). A charged glutamic acid within the transmembrane segment may mediate association with other signaling proteins. Alternative splicing may generate an isoform that lacks the transmembrane segment and an isoform that largely consists of the first two Ig-like domains (5). Mouse FCRL1 contains only two Ig-like domains, but it shares 62% aa sequence identity with homologous regions of the human FCRL1 ECD. FCRL1 is expressed on pre-B cells and naive B cells (6, 7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).

1. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
3. Miller, I. et al. (2002) Blood 99:2662.
4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
5. Accession # Q96LA6.
6. Leu, C.-M. et al. (2005) Blood 105:1121.
7. Li, F.J. et al. (2008) Blood 112:179.
8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
9. Du, X. et al. (2008) Blood 111:338.
10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
11. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCRL1
• Uniprot:
  • Q96LA6()