FCP1 Antibody

Product Discontinued

FCP1 Antibody Summary

• Immunogen: Synthetic peptide: EVPAAGRVPA representing the N-Terminus of the human protein (residues 2-11), according to NP_004706; NP_430255.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTDP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2-4 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA 1:16000
• Application Notes: Western Blot: Preliminary experiments gave no signal but low background in Human Kidney and Placenta lysates at upto 1ug/ml. Expected to recognise both reported isoforms (represented by NP_004706 and NP_430255). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Reactivity Notes

Cross reacts with Human. Expected from sequence similarity: Mouse, Rat and Dog.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for FCP1 Antibody

• CCFDN
• CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase
• CTD of POLR2A, phosphatase of, subunit 1
• EC 3.1.3.16
• FCP1TFIIF-associating CTD phosphatase 1
• RNA polymerase II subunit A C-terminal domain phosphatase
• serine phosphatase FCP1a
• subunit 1
• TFIIF-associating CTD phosphatase
• transcription factor IIF-associating CTD phosphatase 1

Background

This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTDP1
• Entrez:
  • 9150(Human)
• Uniprot:
  • NP_004706(Human)