FATP2/SLC27A2 Knockout HeLa Cell Lysate

Product Discontinued

FATP2/SLC27A2 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-2 bp deletion in exon1 and -1 bp deletion in exon1 and 82 bp insertion in exon1
• Gene: SLC27A2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for FATP2/SLC27A2 Knockout HeLa Cell Lysate

• ACSVL1
• ACSVL1FATP-2
• EC 6.2.1
• EC 6.2.1.-
• EC 6.2.1.3
• EC 6.2.1.7
• FACVL1
• FACVL1very long-chain fatty-acid-coenzyme A ligase 1
• FATP2
• FATP2THCA-CoA ligase
• Fatty acid transport protein 2
• Fatty-acid-coenzyme A ligase, very long-chain 1
• hFACVL1
• HsT17226
• Long-chain-fatty-acid--CoA ligase
• SLC27A2
• solute carrier family 27 (fatty acid transporter), member 2
• Solute carrier family 27 member 2
• THCA-CoA ligase
• very-long-chain acyl-CoA synthetase
• VLACS
• VLACSVery long-chain-fatty-acid-CoA ligase
• VLCS
• VLCSvery long-chain acyl-CoA synthetase

Background

The protein encoded by this gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme activates long-chain, branched-chain and very-long-chain fatty acids containing 22 or more carbons to their CoA derivatives. It is expressed primarily in liver and kidney, and is present in both endoplasmic reticulum and peroxisomes, but not in mitochondria. Its decreased peroxisomal enzyme activity is in part responsible for the biochemical pathology in X-linked adrenoleukodystrophy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SLC27A2